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Development of a Monoclonal Antibody-Based Immunochromatographic Assay Detecting Ractopamine Residues in Swine Urine

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Abstract

A lateral-flow immunochromatographic assay (LFIA) using monoclonal antibody was developed for the rapid detection of ractopamine residues in swine urine. The assay procedure could be accomplished within 5 min, and the results of this qualitative one-step assay were evaluated visually according to whether test lines appeared or not. When applied to the swine urines, the detection limit and the half maximal inhibitory concentration (IC50) of the lateral-flow immunochromatographic test strip under an optical density scanner were calculated to be 0.1 ± 0.013 and 0.71 ± 0.056 ng/mL, respectively. The cut-off level was observed with the naked eye of 1 ng/mL for detection of ractopamine residue in swine urine. Parallel analysis of swine urine samples with ractopamine showed comparable results obtained from the LFIA and LC-MS/MS. Therefore, the described lateral-flow test strip can be used as a reliable, rapid and cost-effective on-site screening technique for the determination of ractopamine residues in swine urine.

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Correspondence to Mingzhou Zhang.

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Funding

This study was funded by the Natural Science Foundation Programs of Zhejiang Province (LY13C200009), the Important Science and Technology Specific Program of Zhejiang Province (2012C12013-3) and the Key Project in Agriculture of Ningbo (2013C11003).

Conflict of Interest

Mingzhou Zhang has received research grants from Zhejiang Province (LY13C200009, 2012C12013-3) and Ningbo City (2013C11003). Amin Liu declares that she has no conflict of interest. Junxiao Lin declares that he has no conflict of interest. Mingyan Dai declares that she has no conflict of interest. Bio Ma declares that he has no conflict of interest. Yingying Wu declares that she has no conflict of interest. Jiehong Fang declares that he has no conflict of interest. Mingzhou Zhang declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants performed by any of the authors. All animal studies were carried out under the guidance of animal welfare committee of Hangzhou Normal University.

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Liu, A., Lin, J., Dai, M. et al. Development of a Monoclonal Antibody-Based Immunochromatographic Assay Detecting Ractopamine Residues in Swine Urine. Food Anal. Methods 9, 2016–2025 (2016). https://doi.org/10.1007/s12161-015-0382-5

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  • DOI: https://doi.org/10.1007/s12161-015-0382-5

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