Abstract
A simple method using dispersive liquid-liquid microextraction (DLLME) combined with capillary zone electrophoresis (CZE) was developed for detection and quantification of vanillin in milk powder. CZE analytical conditions and DLLME parameters were optimized. The optimal running buffer was 10 mM borate buffer at pH 8.0. 4-Hydroxybenzaldehyde was used as the internal standard. The optimized microextraction conditions were 5.0 mL sample solution at pH 5.5, 8 % (m/v) NaCl added, with 120 μL CHCl3 (extraction solvent), and 0.8 mL tetrahydrofuran (THF) (disperser solvent) injected. Under the optimum extraction and detection conditions, vanillin was determined in 5 min, with the limit of detection at 0.02 μg mL−1 and a quantitation range of 0.05–10 μg mL−1. The method was successfully applied to the analysis of vanillin in infant milk powder samples. The recoveries obtained were between 89.7 and 95.1 % with relative standard deviation (RSD) less than 2.5 %.
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We are grateful for the financial support from the National Natural Science Foundation of China (No. 51172195).
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Ming Shu declares that she has no conflict of interest. Yanru Man declares that she has no conflict of interest. Hui Ma declares that she has no conflict of interest. Feng Luan declares that he has no conflict of interest. Huitao Liu declares that she has no conflict of interest. Yuan Gao declares that he has no conflict of interest.
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Shu, M., Man, Y., Ma, H. et al. Determination of Vanillin in Milk Powder by Capillary Electrophoresis Combined with Dispersive Liquid-Liquid Microextraction. Food Anal. Methods 9, 1706–1712 (2016). https://doi.org/10.1007/s12161-015-0347-8
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DOI: https://doi.org/10.1007/s12161-015-0347-8