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Rapid Assessment of Fish Freshness and Quality by 1H HR-MAS NMR Spectroscopy

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Abstract

A new analytical method that allows the rapid assessment of fish freshness and quality is presented. The method is based on 1H high-resolution magic angle spinning (HR-MAS) NMR spectroscopy and allows the rapid determination of two well-established indicators of fish freshness and quality: the K value and the trimethylamine nitrogen (TMA-N) content. The method is demonstrated on four different species of fish (sea bream, sea bass, trout, and red mullet) stored on ice at 0 °C. The results obtained are in agreement with more cumbersome methods classically used to determine the K value and the TMA-N concentration. The main advantage of the 1H HR-MAS NMR approach is to allow a direct measurement of these two parameters directly on unprocessed fish sample without using any preliminary extraction. The total analysis time, including sample preparation, is of the order of 40 min per sample.

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Acknowledgments

This work is part of the AgriFood GPS project (“http://www.eurofins.fr/fr-fr/actualites/actualites/agrifood-gps.aspx”) and was supported in part by Bpifrance (formerly Oséo) and Bruker BioSpin France.

Conflict of Interest

Clément Heude declares that he has no conflict of interest. Elise Lemasson declares that she has no conflict of interest. Karim Elbayed declares that he has no conflict of interest. Martial Piotto declares that he has no conflict of interest. All institutional and national guidelines for the care and use of laboratory animals were followed.

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Authors and Affiliations

  1. Bruker BioSpin, 34 rue de l’industrie, 67166, Wissembourg, France

    C. Heude, E. Lemasson & M. Piotto

  2. IMIS/ICube Laboratory, Strasbourg University, 67000, Strasbourg, France

    C. Heude, K. Elbayed & M. Piotto

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  1. C. Heude
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  2. E. Lemasson
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  3. K. Elbayed
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  4. M. Piotto
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Correspondence to M. Piotto.

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Heude, C., Lemasson, E., Elbayed, K. et al. Rapid Assessment of Fish Freshness and Quality by 1H HR-MAS NMR Spectroscopy. Food Anal. Methods 8, 907–915 (2015). https://doi.org/10.1007/s12161-014-9969-5

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  • DOI: https://doi.org/10.1007/s12161-014-9969-5

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