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A Voltammetric Sensor for Simultaneous Determination of Vitamin C and Vitamin B6 in Food Samples Using ZrO2 Nanoparticle/Ionic Liquids Carbon Paste Electrode

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Abstract

A fast and simple voltammetric method based on the use of ZrO2 nanoparticle/ionic liquids carbon paste electrode (ZrO2/NPs/IL/CPE) is proposed for the determination of vitamin C (ascorbic acid (AA)) in the presence of vitamin B6. In the first step, we describe synthesis and characterization of ZrO2 nanoparticles (ZrO2/NPs) with different methods such as transmission electron microscopy (TEM) and X-ray diffraction (XRD). In the second step, application of the synthesis nanoparticles describes for the preparation of carbon paste electrode modified with 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim]BF4) as a binder for voltammetric determination of AA and vitamin B6 in food samples. At an optimum condition (pH 7.0), the two peaks are separated ca. 0.44 and 0.82 V for AA and vitamin B6. This point shows that AA can be determined in the presence of vitamin B6 and more than 3.75 times current excess of AA. The peaks current of square wave voltammograms (SWV) of AA and vitamin B6 increased linearly with their concentration in the ranges of 0.07–850 μM AA and 0.8–550 μM vitamin B6. The detection limits for AA and vitamin B6 were 0.009 and 0.1 μM, respectively. The modified electrode has been successfully applied for the assay of AA and vitamin B6 in food samples such as tablet, urine, fruit juice, and injection in comparison with other method. The obtained data was check with F and t tests.

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Acknowledgments

The authors wish to thank Graduate University of Advanced Technology, Kerman, Iran, for their support.

Conflict of Interest

Amin Baghizadeh has no conflict of interest. Hassan Karimi-Maleh has no conflict of interest. Zahra Khoshnama has no conflict of interest. Asadollah Hassankhani has no conflict of interest. Maryam Abbasghorbani has no conflict of interest. This work did not receive support from the university, and research has been conducted in the Laboratory of Nano Sciences in Graduate University of Advanced Technology, Kerman, Iran. Finally, this article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran

    Amin Baghizadeh

  2. Department of Chemistry, Graduate University of Advanced Technology, Kerman, Iran

    Hassan Karimi-Maleh

  3. Department of Physics, Mazandaran Science and Research Branch, Islamic Azad University, Mazandaran, Iran

    Zahra Khoshnama

  4. Department of Materials Science Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran

    Asadollah Hassankhani

  5. Gas Division, Research Institute of Petroleum Industry, P.O. Box 14665-137, Tehran, Iran

    Maryam Abbasghorbani

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  1. Amin Baghizadeh
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  2. Hassan Karimi-Maleh
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  3. Zahra Khoshnama
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  4. Asadollah Hassankhani
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Correspondence to Hassan Karimi-Maleh.

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Baghizadeh, A., Karimi-Maleh, H., Khoshnama, Z. et al. A Voltammetric Sensor for Simultaneous Determination of Vitamin C and Vitamin B6 in Food Samples Using ZrO2 Nanoparticle/Ionic Liquids Carbon Paste Electrode. Food Anal. Methods 8, 549–557 (2015). https://doi.org/10.1007/s12161-014-9926-3

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