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Phytochemical Characterization of Phagnalon graecum Boiss. by HPLC and GC-MS with its Enzyme Inhibitory and Antioxidant Activity Profiling by Spectrophotometric Methods

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Abstract

In the current study, the n-hexane, chloroform, ethyl acetate, methanol, and water extracts of Phagnalon graecum Boiss. (Asteraceae), a spice plant, were investigated for their enzyme inhibitory activity against acetylcholinesterase, butyrylcholinesterase, lipoxygenase, tyrosinase, and antioxidant activities. Antioxidant activity of the extracts was determined by 2,2-diphenyl-1-picrylhydrazyl radical scavenging, ferric ion-chelation activity, and ferric-reducing antioxidant power tests. Total phenol (gallic acid equivalent) and flavonoid (quercetin equivalent) contents were calculated. Essential oil of the plant was analyzed by GC-MS, while the selected phenolic acids were analyzed by HPLC. The extracts had insignificant inhibition against the tested enzymes, whereas they displayed a remarkable antioxidant activity. Therefore, to the best of our knowledge, this study presents the first data on biological activity and phytochemical content of P. graecum and this species could be utilized as potential antioxidant applicable for food preservation.

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Acknowledgment

F.S. Senol expresses her genuine gratitude to the Scientific and Technological Research Council of Turkey (TUBITAK) for the scholarship provided during her Ph.D. program.

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Authors and Affiliations

  1. Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330, Ankara, Turkey

    Ilkay Erdogan Orhan, Fatma Sezer Senol & Bilge Sener

  2. Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Eastern Mediterranean University, Famagusta, Northern Cyprus, Turkey

    Ilkay Erdogan Orhan

  3. Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, 26470, Eskisehir, Turkey

    Betul Demirci, Nilgun Ozturk & Kemal Husnu Can Baser

  4. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia

    Kemal Husnu Can Baser

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  1. Ilkay Erdogan Orhan
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  2. Fatma Sezer Senol
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  3. Betul Demirci
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  4. Nilgun Ozturk
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  5. Kemal Husnu Can Baser
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  6. Bilge Sener
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Correspondence to Ilkay Erdogan Orhan.

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Erdogan Orhan, I., Senol, F.S., Demirci, B. et al. Phytochemical Characterization of Phagnalon graecum Boiss. by HPLC and GC-MS with its Enzyme Inhibitory and Antioxidant Activity Profiling by Spectrophotometric Methods. Food Anal. Methods 6, 1–9 (2013). https://doi.org/10.1007/s12161-012-9386-6

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  • DOI: https://doi.org/10.1007/s12161-012-9386-6

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