Abstract
The sequence-specific backbone assignment of the mitogen-activated protein kinase (MAPK) binding domain of the dual-specificity phosphatase 1 (DUSP1) has been accomplished using a uniformly [13C, 15N]-labeled protein. These assignments will facilitate further studies of DUSP1 in the presence of inhibitors/ligands to target MAPK associated diseases and provide further insights into the function of dual-specificity phosphatase 1 in MAPK regulation.
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Acknowledgements
The authors thank Dr. Heiko Zettl for help at the early stages of the project. This research was supported by Grant RSG-08-067-01-LIB from the American Cancer Society to R.P. and by R01GM100910 from the National Institute of Health to W.P. This research is based in part on data obtained at the Brown University Structural Biology Core Facility, which is supported by the Division of Biology and Medicine, Brown University. 800 MHz NMR data were recorded at Brandeis University.
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Kumar, G.S., Page, R. & Peti, W. 1H, 15N and 13C sequence specific backbone assignment of the MAP kinase binding domain of the dual specificity phosphatase 1 and its interaction with the MAPK p38. Biomol NMR Assign 15, 243–248 (2021). https://doi.org/10.1007/s12104-021-10012-z
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DOI: https://doi.org/10.1007/s12104-021-10012-z