Abstract
We report the 94 % assignment of DVU2108, a protein belonging to the Orange Protein family, that in Desulfovibrio vulgaris Hildenborough forms a protein complex named the Orange Protein complex. This complex has been shown to be implicated in the cell division of this organism. DVU2108 is a conserved protein in anaerobic microorganisms and in Desulfovibrio gigas the homologous protein was isolated with a novel Mo–Cu cluster non-covalently attached to the polypeptide chain. However, the heterologously produced DVU2108 did not contain any bound metal. These assignments provide the means to characterize the interaction of DVU2108 with the proteins that form the Orange Protein complex using NMR methods.
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Acknowledgments
This work was supported by a research project from the Fundação para a Ciência e Tecnologia (FCT-ANR/BBB-MET/0023/2012) to SRP, which also supported the NMR spectrometer (RECI/BBB-BQB/0230/2012), which is part of the National NMR Network. MSPC would like to thank CAPES-BJT programme for financial support.
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Neca, A.J., Soares, R., Carepo, M.S.P. et al. Resonance assignment of DVU2108 that is part of the Orange Protein complex in Desulfovibrio vulgaris Hildenborough. Biomol NMR Assign 10, 117–120 (2016). https://doi.org/10.1007/s12104-015-9648-5
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DOI: https://doi.org/10.1007/s12104-015-9648-5