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High serum microRNA-335 level predicts aggressive tumor progression and unfavorable prognosis in pediatric acute myeloid leukemia

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Abstract

Purpose

MiRNA expression profiles previously showed the higher expression of microRNA(miR)-335 in bone marrow samples of pediatric acute myeloid leukemia (AML) patients than normal controls. Our aim was to investigate associations of miR-335 expression with tumor progression and prognosis in pediatric AML.

Methods

Real-time quantitative PCR was performed to detect the expression of miR-335 in bone marrow mononuclear cells and serum obtained from patients with pediatric AML and healthy controls.

Results

Expression levels of miR-335 in the bone marrow and serum of pediatric AML patients were both significantly higher than those in normal controls (both P < 0.001). Then, high serum miR-335 level occurred more frequently in French-American-British classification subtype M7 subtype than in other subtypes (P = 0.03). The expression of serum miR-335 in pediatric AML patients with unfavorable karyotypes was also significantly higher than those in intermediate and favorable groups (P = 0.008). Moreover, high serum miR-335 level was markedly associated with shorter relapse-free and overall survivals (both P < 0.001) of patients with pediatric AML. Furthermore, the multivariate analysis identified the serum miR-335 and cytogenetics risk as independent prognostic factors for both relapse-free and overall survivals. More importantly, the prognostic relevance of serum miR-335 expression was more obvious in the subgroup of patients with intermediate-risk cytogenetics.

Conclusion

Our data offer the convincing evidence for the first time that serum miR-335 level may be markedly and consistently increased in pediatric AML patients. Serum miR-335 may serve as a promising marker for monitoring the progression and predicting the clinical outcome of patients with this disease.

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Conflict of interest

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Authors and Affiliations

  1. Department of Paediatric Intensive Care Units, Huai’an Maternity and Child Health Hospital of Yangzhou University, Huai’an, 223002, Jiangsu Province, China

    X. Lin

  2. Department of Pediatrics, Huai’an Hospital Affiliated of Xuzhou Medical College and Huai’an Second People’s Hospital, 62 Huaihai Road South, Huai’an, 223002, China

    Z. Wang

  3. Department of Pediatrics, Huai’an First People’s Hospital, Nanjing Medical University, 6 Beijing Road West, Huai’an, 220300, Jiangsu, China

    R. Zhang & W. Feng

Authors
  1. X. Lin
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  2. Z. Wang
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  3. R. Zhang
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  4. W. Feng
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Corresponding author

Correspondence to W. Feng.

Additional information

X. Lin and Z. Wang contributed equally to this work.

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Lin, X., Wang, Z., Zhang, R. et al. High serum microRNA-335 level predicts aggressive tumor progression and unfavorable prognosis in pediatric acute myeloid leukemia. Clin Transl Oncol 17, 358–364 (2015). https://doi.org/10.1007/s12094-014-1237-z

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  • DOI: https://doi.org/10.1007/s12094-014-1237-z

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