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Optimisation of Manganese Peroxidase (MnP) Activity of Enterobacter wuhouensis Using Response Surface Method and Evaluation of Its Maillard Reaction Products Along with Lignin Degradation Ability

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Abstract

Manganese peroxidase (MnP), a microbial ligninolytic enzyme which plays significant role in lignin and melanoidin degradation has gained much attention in the field of industry. In the present study, 15 ligninolytic bacteria were isolated from the soil sample of Similipal Biosphere Reserve (SBR) and screened for MnP activity. The most efficient MnP-producing bacterium HNB5 was evaluated for alkali lignin and maillard reaction products (MRPs) degradation and identified as Enterobacter wuhouensis using 16S rRNA sequencing. This bacterium exhibited the highest MnP activity of 2.6 U mL−1 min−1 in un-optimized conditions. Further, optimization using response surface methodology E. wuhouensis showed increased MnP activity of 4.11 U mL−1 min−1 at pH 6.3, temperature 37 °C, substrate concentration 1.05%, and time 144 h. In both FT-IR and UV–Vis spectrophotometry analyses of control and bacterium degraded MRPs, the reduction in Maillard product colour was correlated with shifting absorption peaks. Also, the GC–MS analysis data showing a change in functional group revealed the rise of novel peaks caused due to the degradation of MRPs complex. The phytotoxicity study was conducted for bacterial degraded MRPs medium revealed that toxicity of the medium decreased after bacterial treatment. The findings of the current study suggest that the manganese MnP produced by E. wuhouensis isolated from SBR soil sample may be employed for bioremediation purposes to degrade MRPs.

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Acknowledgements

Authors are grateful to the HOD of Department of Biotechnology, Maharaja Sriram Chandra Bhanja Deo University, Baripada, Odisha, India for providing infrastructure facilities to conduct the present work. The author(s) are grateful to AGL, IIT (ISM), Dhanbad for FTIR analyses, and visualization(s) used in this study. The author(s) are also grateful to Dr. Rajiv Chandra Dev Goswami, Research associate, Guwahati Biotech Park, Science Technology and Climate Change Department, Amingaon , Guwahati for providing technical support during GC-MS analysis used in this study.

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  1. Department of Biotechnology, Maharaja Sriram Chandra Bhanja Deo University, Takatpur, Baripada, Odisha, India

    Hrudayanath Thatoi, Subhashree Rath & Nitish Kumar Kheti

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  1. Hrudayanath Thatoi
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HT developed and supervised the entire research project and made over all editing in manuscript. In this work, SR and NK executed experiments and analyzed the data, as well as helped in writing of the manuscript.

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Correspondence to Hrudayanath Thatoi.

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Thatoi, H., Rath, S. & Kheti, N.K. Optimisation of Manganese Peroxidase (MnP) Activity of Enterobacter wuhouensis Using Response Surface Method and Evaluation of Its Maillard Reaction Products Along with Lignin Degradation Ability. Indian J Microbiol 63, 604–620 (2023). https://doi.org/10.1007/s12088-023-01120-6

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