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Catalase from the Antarctic Fungus Aspergillus fumigatus I-9–Biosynthesis and Gene Characterization

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Abstract

Extremely cold habitats are a serious challenge for the existing there organisms. Inhabitants of these conditions are mostly microorganisms and lower mycetae. The mechanisms of microbial adaptation to extreme conditions are still unclear. Low temperatures cause significant physiological and biochemical changes in cells. Recently, there has been increasing interest in the relationship between low-temperature exposure and oxidative stress events, as well as the importance of antioxidant enzymes for survival in such conditions. The catalase is involved in the first line of the cells' antioxidant defense. Published information supports the concept of a key role for catalase in antioxidant defense against cold stress in a wide range of organisms isolated from the Antarctic. Data on representatives of microscopic fungi, however, are rarely found. There is scarce information on the characterization of catalase synthesized by adapted to cold stress organisms. Overall, this study aimed to observe the role of catalase in the survival strategy of filamentous fungi in extremely cold habitats and to identify the gene encoded catalase enzyme. Our results clearly showed that catalase is the main part of antioxidant enzyme defense in fungal cells against oxidative stress caused by low temperature exposure.

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Authors and Affiliations

  1. Departament of Mycology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G, Bonchev Str. Bl.26, 1113, Sofia, Bulgaria

    Vladislava Dishliyska, Radoslav Abrashev, Jeny Miteva-Staleva, Boriana Spasova, Maria Angelova & Ekaterina Krumova

  2. Departament of General Microbiology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G, Bonchev Str. Bl.26, 1113, Sofia, Bulgaria

    Galina Stoyancheva

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  1. Vladislava Dishliyska
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Dishliyska, V., Stoyancheva, G., Abrashev, R. et al. Catalase from the Antarctic Fungus Aspergillus fumigatus I-9–Biosynthesis and Gene Characterization. Indian J Microbiol 63, 541–548 (2023). https://doi.org/10.1007/s12088-023-01110-8

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