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Promoted Spore Formation of Bacillus amyloliquefaciens fmbJ by its Secondary Metabolite Bacillomycin D Coordinated with Mn2+

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Abstract

In Bacillus, the spore formation process is associated with the synthesis and release of secondary metabolites. A large number of studies have been conducted to systematically elucidate the pathways and mechanisms of spore formation. However, there are no studies have explored the relationship between secondary metabolites and spores. In this study, we investigated the relationship between its secondary metabolite bacillomycin D (BD) and spores using the simpler dipicolonic acid fluorimetry assay for spore counting in Bacillus amyloliquefaciens fmbJ. Our results showed that BD could promote the spore formation of B. amyloliquefaciens fmbJ and had a synergistic effect with certain concentrations of Mn2+. When 15.6 mg/L of BD and 1 mM of Mn2+ were added, the number of fmbJ spores increased from 1.42 × 108 CFU/mL to 2.02 × 108 CFU/mL after 36 h of incubation. The expressions of spore formation (kinA, kinB, kinC, kinD, kinE and spo0A) and Mn-related genes (mntA, mntH, mneS, mneP) were studied by RT-PCR. The results indicated that BD and Mn2+ promoted the spore formation of fmbJ by stimulating the transcription of kinB, kinD and increasing the influence of spo0F-spo0A phosphorylation transmission. This study provided a new idea to improve the spore production of B. amyloliquefaciens and laid the foundation for its industrial production.

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The data used to support the findings of this study are available from the corresponding author upon request.

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Acknowledgements

This work was financially supported by grants from the Natural Science Foundation of Jiangsu Province-China (BK20200835) and the Priority Academic Program Development of Jiangsu Higher Education Institutions-China (PAPD).

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Authors and Affiliations

  1. College of Food Science and Engineering/Collaborative, Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, 3 Wenyuan Road, Xianlin University Town, Nanjing, 210023, Jiangsu Province, People’s Republic of China

    Jin Zhang, Xiaojiao Luo, Xinyi Pang, Xiangfei Li, Yingjian Lu & Jing Sun

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  1. Jin Zhang
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Contributions

Jing Sun and Yingjian Lu conceived and designed research. Jin Zhang and Xiaojiao Luo conducted experiments. Jing Sun and Jin Zhang analyzed data and wrote the manuscript. Xinyi Pang and Xiangfei Li revised the manuscript. All authors read and approved the manuscript.

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Correspondence to Yingjian Lu or Jing Sun.

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Zhang, J., Luo, X., Pang, X. et al. Promoted Spore Formation of Bacillus amyloliquefaciens fmbJ by its Secondary Metabolite Bacillomycin D Coordinated with Mn2+. Indian J Microbiol 62, 531–539 (2022). https://doi.org/10.1007/s12088-022-01026-9

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