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Genotyping of Antimicrobial Resistance and Virulence in Staphylococcus Isolated from Food of Animal Origin in Mexico

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Abstract

Ninety-six methicillin-susceptible Staphylococcus aureus (MSSA) and 11 methicillin-resistant coagulase-negative staphylococci (MRCNS) were recovered from food of animal origin. Multi-drug resistance was detected in 34.1% of isolates. Tetracycline-resistant staphylococci harbored tetK gene (68.8%). Erythromycin/clindamycin-resistant staphylococci carried lnuA/lnuB genes frequently alone or combined with msrA gene. The sec gene was detected in 15.6% of MSSA and two isolates harbored the immune evasion cluster. The spa t337 predominated among MSSA strains. Two ermC-positive MRCNS isolates were observed, five mecA-positive carried SCCmec IVa and 6 were non-typeable by the IWG-SCC classification. These results demonstrate that food of animal origin can be a potential source for spreading of multidrug-resistance gene.

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Acknowledgements

We thank German González-Mago Ph.D. and Gerardo Cortés-Cortés Ph.D., for the support in reviewing the manuscript. This work was supported by CONACYT México (Reference 178942). Yosainix C. Gaerste-Diaz had a scholarship from CONACYT (No. 104293).

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Authors and Affiliations

  1. Instituto de Ciencias, Posgrado en Microbiología. Centro de Investigaciones en Ciencias Microbiológicas. Edificio IC-11. Ciudad Universitaria. Colonia San Manuel, Benemérita Universidad Autónoma de Puebla, 72570, Puebla, Mexico

    Yosainix C. Gaerste-Díaz, Patricia Lozano-Zarain & Rosa del C. Rocha-Gracia

  2. Área Bioquímica y Biología Molecular, Universidad de la Rioja, Madre de Dios 51, 26006, Logroño, La Rioja, Spain

    Carmen Torres

  3. Facultad de Ingeniería Agrohidráulica, Benemérita Universidad Autónoma de Puebla, Av, Universidad s/n, Junta Auxiliar de San Juan Acateno, Teziutlán, CP. 73695, Puebla, Mexico

    Numa P. Castro González

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  1. Yosainix C. Gaerste-Díaz
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  2. Patricia Lozano-Zarain
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  3. Carmen Torres
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  4. Numa P. Castro González
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  5. Rosa del C. Rocha-Gracia
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Corresponding author

Correspondence to Rosa del C. Rocha-Gracia.

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Gaerste-Díaz, Y.C., Lozano-Zarain, P., Torres, C. et al. Genotyping of Antimicrobial Resistance and Virulence in Staphylococcus Isolated from Food of Animal Origin in Mexico. Indian J Microbiol 58, 525–528 (2018). https://doi.org/10.1007/s12088-018-0745-x

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