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Effect of Nitric Oxide on the Antifungal Activity of Oxidative Stress and Azoles Against Candida albicans

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Abstract

Nitric oxide (NO) is a small molecule with a wide range of biological activities in mammalian and bacteria. However, the role of NO in fungi, especially Candida albicans, is not clear. In this study, we confirmed the generation of endogenous NO in C. albicans, and found that the production of endogenous NO in C. albicans was associated with nitric oxide synthase pathway. Our results further indicated that the production of endogenous NO in C. albicans was reduced under oxidative stress such as menadione or H2O2 treatment. Meanwhile, exogenous NO donor, sodium nitroprusside (SNP), synergized with H2O2 against C. albicans. Interestingly, SNP could inhibit the antifungal effect of azoles against C. albicans in vitro, suggesting that NO might be involved in the resistance of C. albicans to antifungals. Collectively, this study demonstrated the production of endogenous NO in C. albicans, and indicated that NO may play an important role in the response of C. albicans to oxidative stress and azoles.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (81573724 and 81501730) and the Shanghai Pujiang Program (14PJD001).

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    Authors and Affiliations

    1. Department of Clinical Pharmacology, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853, China

      De-Dong Li, Ping Liu & Yan Sun

    2. New Drug Research and Development Center, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai, 200433, China

      De-Dong Li & Yan Wang

    3. The First Department of South Building, General Hospital of Chinese Armed Police Forces, Beijing, 100039, China

      Chang-Chun Yang

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    1. De-Dong Li
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    Correspondence to Yan Wang or Yan Sun.

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    De-Dong Li and Chang-Chun Yang have contributed equally to this work.

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    Li, DD., Yang, CC., Liu, P. et al. Effect of Nitric Oxide on the Antifungal Activity of Oxidative Stress and Azoles Against Candida albicans . Indian J Microbiol 56, 214–218 (2016). https://doi.org/10.1007/s12088-016-0580-x

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