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Regulation of Saccharomyces cerevisiae Plasma membrane H+-ATPase (Pma1) by Dextrose and Hsp30 during Exposure to Thermal Stress

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Abstract

Pma1p is an essential plasma membrane H+-pump in Saccharomyces cerevisiae that pumps out H+ at the expense of cellular ATP. Its activity is induced by glucose at 30°C and is inhibited by Hsp30 during exposure to heat shock conditions. To further investigate the regulation of Pma1 function by glucose and Hsp30 during exposure to thermal stress, we estimated Pma1 activity, its protein levels and ser-phosphorylation status in membrane fractions isolated from BY4741 and hsp30Δ cells grown in dextrose and sorbitol at 30°C, and following exposure at 40°C for 30 min. Our results demonstrate that Pma1 activity and protein levels were reduced in Hsp30+ cells following exposure to thermal stress in dextrose media. The above was not observed in hsp30Δ cells wherein Pma1 activity did not decrease following exposure to similar conditions. Although Pma1p levels decreased in heat-shocked hsp30Δ cells, it was lower compared to that observed in Hsp30+ cells. Total ser-phosphorylation of Pma1 also showed a decrease following exposure to heat shock condition in dextrose media in both BY4741 and hsp30Δ cells. Its levels were also reduced in BY4741 cells upon heat shock treatment in sorbitol unlike that observed in hsp30Δ cells wherein it was increased. Taken together the above indicate that heat shock induced reduction in Pma1 activity and protein levels in dextrose media required Hsp30. To examine functional interactions between dextrose utilization, Hsp30 and the regulation of various aspects of Pma1, we determined if dextrose regulated other functions attributed to Hsp30. Results demonstrate that the deletion of HSP30 rendered cells dependent on dextrose utilization for survival during exposure to lethal heat stress. Our study has hence been able to establish a functional relationship between glucose utilization, Hsp30 function and the regulation of Pma1 activity. Finally, since the deletion of HSP30 renders Pma1p levels and its activity unresponsive to thermal stress in dextrose media, we concluded that Hsp30 is necessary to maintain Pma1 in a regulation competent conformation. Hsp30 may thus act as a chaperone in the S. cerevisiae plasma membrane.

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Acknowledgments

We are grateful to Dr. G. Ilavazhagan Director, DIPAS, for his support during the course of this work. S. cerevisiae BY4741 was obtained from the laboratory of Dr. A. K. Bachhawat, Institute of Microbial Technology, Chandigarh. The work was supported by the Ministry of Defence, Govt. of India. ST a former research fellow was supported by a fellowship from DRDO.

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  1. Suresh Thakur

    Present address: Triesta Sciences India Pvt. Ltd., Bangalore, Karnataka, 560027, India

Authors and Affiliations

  1. Department of Molecular Biology, Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi, 110054, India

    Ramesh C. Meena, Suresh Thakur & Amitabha Chakrabarti

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  1. Ramesh C. Meena
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  2. Suresh Thakur
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Correspondence to Amitabha Chakrabarti.

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Meena, R.C., Thakur, S. & Chakrabarti, A. Regulation of Saccharomyces cerevisiae Plasma membrane H+-ATPase (Pma1) by Dextrose and Hsp30 during Exposure to Thermal Stress. Indian J Microbiol 51, 153–158 (2011). https://doi.org/10.1007/s12088-011-0137-y

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