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CircRNA hsa_circ_0001627 aggravates cervical cancer progression through upregulation of FNDC3B and activating PI3K/mTOR signaling pathway

  • Research article
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Journal of Cell Communication and Signaling Aims and scope

Abstract

Circular RNAs (CircRNAs) are key regulators in the development and progression of human cancers. However, the biological roles and mechanisms of circRNAs in gastric cancer (GC) remain largely unknown. Analyzing circRNA microarray dataset (GSE102686) and clinical specimens, a novel circRNA termed hsa_circ_0001627, was identified and it was highly expressed in CC cancerous tissues and cells, and was associated with poor clinical outcomes. Functionally, hsa_circ_0001627 silencing impaired the malignant progression of CC cells and the growth of CC xenografts in nude mice. Mechanistically, hsa_circ_0001627 acted as a miR-1225-5p sponge, thus indirectly regulating FNDC3B and leading to the activation of PI3K/mTOR signaling pathway. Collectively, the present study indicates that hsa_circ_0001627 regulates miR-1225-5p/FNDC3B/PI3K/mTOR axis and functions as an oncogene in CC progression, suggesting the potential therapeutic use of hsa_circ_0001627 in CC treatment.

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Data Availability

All data generated or analyzed during this study are included in this published article.

Abbreviations

CC:

Cervical cancer

ceRNA:

Competitive endogenous RNA

CircRNA:

Circular RNA

FNDC3B:

Fibronectin type III domain containing 3B

GEO:

Gene Expression Omnibus

H&E:

Hematoxylin and eosin

HPV:

Human papillomavirus

MMP:

Matrix metalloproteinase

PVDF:

Polyvinylidene fluoride

QRT-PCR:

Quantitative real-time PCR

SD:

Standard deviation

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

siRNA:

Small interference RNA

TBST:

Tris buffered saline tween

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Funding

This project was supported by the Liaoning Province science and technology and People’s Livelihood project (2021JH2/10300047).

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Authors and Affiliations

  1. Department of Biotherapy, Cancer Research Institute, The First Hospital of China Medical University, 110001, Shenyang, Liaoning, China

    Yan Li, Fandong Meng, Chengguang Sui & Yang Wang

  2. Department of Gynaecology and Obstetrics, Shengjing Hospital of China Medical University, No. 36, Sanhao Street, Heping District, 110000, Shenyang, Liaoning, China

    Dali Cheng

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  1. Yan Li
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  2. Fandong Meng
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  3. Chengguang Sui
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  4. Yang Wang
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  5. Dali Cheng
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Contributions

YL and DC conceived and designed the experiments, FM and CS analyzed and interpreted the results of the experiments, YW performed the experiments. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Dali Cheng.

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This study was approved by the Ethics Committee of Shengjing Hospital of China Medical University and the Animal Care and Use Committee of Shengjing Hospital of China Medical University.

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All the patients signed written informed consent.

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The authors state that there are no conflicts of interest to disclose.

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Li, Y., Meng, F., Sui, C. et al. CircRNA hsa_circ_0001627 aggravates cervical cancer progression through upregulation of FNDC3B and activating PI3K/mTOR signaling pathway. J. Cell Commun. Signal. 17, 627–638 (2023). https://doi.org/10.1007/s12079-022-00696-w

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  • DOI: https://doi.org/10.1007/s12079-022-00696-w

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