Abstract
The genetics of astringency perception and the contribution of astringency to the pleasantness and use frequency of phenol-rich foods and beverages were studied in a Finnish twin cohort of young adults (aged 22–25 years). A total of 194 participants (96 males, 98 females) comprised of 81 full pairs (24 monozygotic, 57 dizygotic) and 32 twin individuals without the co-twin participating. Pleasantness and intensity of an apple juice with added tannic acid (TA juice; 1.5 g/L) relative to an untainted apple juice were recorded. Two saliva samples were collected, the first after 12 h of fasting and the second after the stimulation with TA juice. Saliva samples were characterized by determining the total protein, amylase, proline-rich protein, histatin, cystatin, and mucin contents. Participants filled in questionnaires comprising of pleasantness and use frequency of eight astringent items vs their less astringent counterparts. TA juice was perceived as less pleasant and more intense than the pure apple juice. The relationship between astringency, product pleasantness, and food use were found to be complex. Although astringency influenced significantly the sensory experience of the phenol-rich foods and beverages, the pleasantness and use frequency of the products were not primarily steered by their astringency level. In twin analyses, the first tentative evidence of the genetic variation underlying astringency perception was discovered.
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Abbreviations
- A/a 2 :
-
Additive genetic effect
- AIC:
-
Akaike’s information criterion
- Am:
-
Amylases
- ANOVA:
-
Analysis of variance
- BSA:
-
Bovine serum albumin
- C/c 2 :
-
Common environmental effect
- CI:
-
Confidence interval
- Cys:
-
Cystatins
- D/d 2 :
-
Dominant genetic effect
- DZ:
-
Dizygotic
- E/e 2 :
-
Unique environmental effect
- His:
-
Histatins
- Muc:
-
Mucins
- MZ:
-
Monozygotic
- PAS:
-
Periodic acid-Schiff
- PROP:
-
6-n-Propylthiouracil
- PRPs:
-
Proline-rich proteins
- R :
-
Rested
- RS:
-
Difference between R and S (R subtracted from S)
- S :
-
Stimulated
- SD:
-
Standard deviation
- SDS:
-
Sodium dodecyl sulfate
- SPTot:
-
Total salivary protein content
- TA juice:
-
Apple juice with added tannic acid
- Tris–HCl:
-
Tris hydrochloride
References
Bajec MR, Pickering GJ (2008) Astringency: mechanisms and perception. Crit Rev Food Sci Nutr 48(9):858–875
Boomsma D, Busjahn A, Peltonen L (2002) Classical twin studies and beyond. Nat Rev Genetics 3(11):872–882
Breslin PAS, Gilmore MM, Beauchamp BK, Green BG (1993) Psychophysical evidence that oral astringency is a tactile sensation. Chem Senses 18(4):405–417
Cala O, Pinaud N, Simon C, Fouquet E, Laguerre M, Dufourc EJ, Pianet I (2010) NMR and molecular modeling of wine tannins binding to saliva proteins: revisiting astringency from molecular and colloidal prospects. FASEB J 24(11):4281–4290
Childs JL, Drake M (2010) Consumer perception of astringency in clear acidic whey protein beverages. J Food Sci 75(9):513–521
De Wijk RA, Prinz JF (2006) Mechanisms underlying the role of friction in oral texture. J Text Stud 37(4):413–427
Dinnella C, Recchia A, Fia G, Bertuccioli M, Monteleone E (2009) Saliva characteristics and individual sensitivity to phenolic astringent stimuli. Chem Senses 34(4):295–304
Dinnella C, Recchia A, Vincenzi S, Tuorila H, Monteleone E (2010) Temporary modification of salivary protein profile and individual responses to repeated phenolic astringent stimuli. Chem Senses 35(1):75–85
Dinnella C, Recchia A, Tuorila H, Monteleone E (2011) Individual astringency responsiveness affects the acceptance of phenol-rich foods. Appetite 56(3):633–642
Glanz K, Basil M, Maibach E, Goldberg J, Snyder D (1998) Why Americans eat what they do: taste, nutrition, cost, convenience, and weight control concerns as influences on food consumption. J Am Diet Assoc 98(10):1118–1126
Green B, Dalton P, Cowart B, Shaffer G, Rankin K, Higgins J (1996) Evaluating the ‘Labeled Magnitude Scale’ for measuring sensations of taste and smell. Chem Senses 21(3):323–334
Hansen JL, Reed DR, Wright MJ, Martin NG, Breslin BA (2006) Heritability and genetic covariation of sensitivity to PROP, SOA, quinine, HCl and caffeine. Chem Senses 31(5):403–413
Joslyn MA, Goldstein JL (1964) Astringency of fruits and fruit products in relation to phenolic content. Adv Food Res 13:179–217
Kallithraka S, Bakker J, Clifford MN (1998) Evidence that salivary proteins are involved in astringency. J Sens Stud 13(1):29–43
Kallithraka S, Bakker J, Clifford MN, Vallis L (2001) Correlations between saliva protein composition and some T-I parameters of astringency. Food Qual Pref 12(2):145–152
Kaprio J, Pulkkinen L, Rose RJ (2002) Genetic and environmental factors in health-related behaviors: studies on Finnish twins and twin families. Twin Res 5(5):366–371
Keskitalo K, Knaapila A, Kallela M, Palotie A, Wessman M, Sammalisto S, Peltonen L, Tuorila H, Perola M (2007) Sweet taste preferences are partly genetically determined: identification of a trait locus on chromosome 16. Am J Clin Nutr 86(1):55–63
Kim UK, Jorgenson E, Coon H, Leppert M, Risch N, Drayna D (2003) Positional cloning of the human quantitative trait locus underlying taste sensitivity to phenylthiocarbamide. Science 299(5610):1221–1225
Lee CB, Lawless HT (1991) Time-course of astringent sensations. Chem Senses 16(3):225–238
Lesschaeve I, Noble AC (2005) Polyphenols: factors influencing their sensory properties and their effects on food and beverage preferences. Am J Clin Nutr 81(1):330–335
Lim J, Lawless HT (2005) Oral sensations from iron and copper sulfate. Physiol Behav 85(3):308–313
Nayak A, Carpenter GH (2008) A physiological model of tea-induced astringency. Physiol Behav 95(3):290–294
Noble AC (2002) Astringency and bitterness of flavonoid phenols. In: Given P, Paredes D (eds) Chemistry of taste: mechanisms, behaviors and mimics. Oxford University Press, Washington, pp 192–201
Peleg H, Gacon K, Schlich P, Noble AC (1999) Bitterness and astringency of flavan-3-ol monomers, dimers and trimers. J Sci Food Agric 79(8):1123–1128
Prinz JF, Lucas PW (2000) Saliva tannin interactions. J Oral Rehab 27(11):991–994
Segrest J, Jackson R (1972) Molecular weight determination of glycoproteins by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. Methods Enzymol 28B:54–63
Tuorila H (2007) Sensory perception as a basis of food acceptance and consumption. In: MacFie H (ed) Consumer-led food product development. Woodhead, Cambridge, pp 34–59
Vidal S, Francis L, Noble AC, Kwiatkowski M, Cheynier V, Waters E (2004) Taste and mouth-feel properties of different types of tannin-like polyphenolic compounds and anthocyanins in wine. Anal Chim Acta 513(1):57–65
Acknowledgments
This work was supported by the University of Helsinki funds, the Finnish Food Research Foundation, the Academy of Finland (grant numbers 100499 and 205585 to J.K.), the Academy of Finland Centre of Excellence in Complex Disease Genetics to J.K. (grant numbers 213506 and 129680), and National Institute of Alcohol Abuse and Alcoholism (grants AA-12502 to R. J. Rose). We thank the twins for participating in the study and Mari Siltala and Eero Vuoksimaa for their excellent assistance in data collection. Authors acknowledge Dr. Simone Vincenzi, C.I.R.V.E., University of Padova, Italy, for electrophoresis analysis.
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Törnwall, O., Dinnella, C., Keskitalo-Vuokko, K. et al. Astringency Perception and Heritability Among Young Finnish Twins. Chem. Percept. 4, 134–144 (2011). https://doi.org/10.1007/s12078-011-9098-0
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DOI: https://doi.org/10.1007/s12078-011-9098-0