Abstract
To develop a molecular pattern that might help in understanding carcinogenesis of postcricoid carcinoma (PCC) on top of Plummer-Vinson syndrome (PVS) in a prospective controlled study. Twenty-four patients with PVS were diagnosed and followed up over a 4 year period, during which eight of them showed malignant change to PCC. Twenty volunteers free of neoplastic diseases were included as a control group. In the two groups, DNA extraction from mononuclear peripheral blood cells, and analysis of loss of heterozygosity (LOH) and microsatellite instability (MSI) using six paired simple tandem repeats (STRs) primers were done. The molecular weight of each STRs locus was scored and statistical correlations were performed. LOH occurred in 55.6 and 72.9% of PVS and PCC cases compared to 25% of control group. At loci D17S695, D9S753 and D9S171, LOH occurred in 54.2, 66.7, and 70.8% of PVS cases; and in 62.5% of PCC cases for each locus compared to 15, 25 and 45% of control cases. D3S1286 and CFS1-R displayed the highest frequency of LOH in PCC (100% for each) while recorded in 58.3 and 33.3% in PVS compared to 30 and 0% in control cases. Certain genetic events tend to occur as early and late events in malignant change of PVS to PCC. Detection of these events may help in understanding carcinogenesis and in early detection of malignancy. CFS1-R is the most informative marker of tumor progression.
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Chen TS, Chen PS (1994) Rise and fall of the Plummer-Vinson syndrome. J Gastroenterol Hepatol 9:654–658
Chrisholm M, Ardran GM, Callander ST, Wright R (1971) Iron deficiency and autoimmunity in postcricoid webs. Q J Med 40:421–433
Jacobs A, Kilpatrick GS (1964) The Patterson-Kelly syndrome. Br Med J 2:79–82
Paterson DR (1919) A clinical type of dysphagia. J Laryngol 24:289–291
Shamma’a MH, Benedict EB (1958) Esophageal webs; a report of 58 cases and an attempt at classification. N Engl J Med 259:378–384
Chisolm M, Ardan GM, Callendar ST, Wright R (1971) A follow-up study of patients with post-cricoid webs. Q J Med 40:409–420
Chisholm M (1974) The association between webs, iron, and post-cricoid carcinoma. Postgrad Med J 50:215–219
Partridge M, Emilion G, Pateromichelakis S, Phillips E, Langdon J (1999) Location of candidate tumour suppressor gene loci at chromosome 3p, 8p, and 9p for oral squamous cell carcinomas. Int J Cancer 83:318–325
Ruppert JM, Tokino K, Sidransky D (1993) Evidence for two bladder cancer suppressor loci on human chromosome 9. Cancer Res 53:5093–5095
Nawroz H, Koch W, Anker P, Stroun M, Sidransky D (1996) Microsatellite alterations in serum DNA of head and neck cancer patients. Nat Med 2:1035–1037
Liu B, Farrington SM, Petersen GM, Hamilton SR, Parsons R, Papadopoulos N, Fujiwara T, Jen J, Kinzler KW, Wyllie AH, Vogelstein B, Dunlop MG (1995) Genetic instability occurs in the majority of young patients with colorectal cancer. Nat Med 1:348–352
Spafford MF, Koch WM, Reed AL, Califano JA, Xu LH, Eisenberger CF, Yip L, Leong PL, Wu L, Liu SX, Jeronimo C, Westra WH et al (2001) Detection of head and neck squamous cell carcinoma among exfoliated oral mucosal cells by micrrosatellite analysis. Clin Cancer Res 7:607–612
Starostik P, Greiner A, Schultz A, Zettl A, Peters K, Rosenwald A, Kolve M, Müller-Hermelink HK (2000) Genetic aberrations common in gastric high-grade large B-cell lymphoma. Blood 95:1180–1187
Partridge M, Kiguwa S, Langdol JD (1994) Frequent deletion of chromosome 3p in oral squamous cell carcinoma. Eur J Cancer Oral Oncol 30B:248–251
Sundaresan V, Ganly P, Hasleton P, Rudd R, Sinha G, Bleehen NM, Rabbitts P (1992) p52 and chromosome 3 abnormalities, characteristic of malignant lung tumours, are detectable in preinvasive lesions of the bronchus. Oncogene 7:1989–1997
Califano J, van der Riet P, Westra W, Nawroz H, Clayman G, Piantadosi S, Corio R, Lee D, Greenberg B, Koch W, Sidransky D (1996) Genetic progression model for head and cancer: implications for field cancerization. Cancer Res 56:2488–2492
Nassar M, Ibrahim SA (2003) A 5-year experience with hypopharyngeal carcinoma in Ain Shams University Hospitals. Inter Cong Ser 1240:1007–1014
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Badawy, B.S., Ahmad, M.AK., Sayed, R.H. et al. Role of Microsatellites Instability in Carcinogenesis of Postcricoid Carcinoma on Top of Plummer-Vinson Syndrome. Indian J Otolaryngol Head Neck Surg 62, 417–420 (2010). https://doi.org/10.1007/s12070-010-0111-8
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DOI: https://doi.org/10.1007/s12070-010-0111-8