Skip to main content
SpringerLink
Log in

Qualitative and quantitative detection of sodium deoxycholic acid in decellularized tissue

  • Original Article
  • Published:
Indian Journal of Thoracic and Cardiovascular Surgery Aims and scope Submit manuscript

Abstract

Background

Decellularization methods using anionic detergents have been applied to engineered tissues for eliminating tissue immunogenicity. Sodium deoxycholate is a water soluble ionic detergent commonly used in applications ranging from decellularisation, liposome preparation and isolation of membrane proteins and lipids. Sodium deoxycholate has been reported to form many reactive intermediates which bind to tissue and exert toxic effects.

Methods

This work reports on methods to detect and estimate residual sodium deoxycholate in decellularized saphenous vein. The method reported is based on the extraction of anionic detergent (sodium deoxycholate)-Methylene blue complex in chloroform which can be subsequently quantified by measuring the optical density at 653 nm.

Results and conclusion

This assay may be used for detecting sodium deoxycholate levels in decellularized tissue before implantation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Gilbert TW, Sellaro TL, Badylak SF. Decellularization of tissues and organs. Biomaterials. 2006;27:3675–83.

    CAS  PubMed  Google Scholar 

  2. Chen F, Yoo JJ, Atala A. Acellular collagen matrix as a possible ‘off the shelf’ biomaterial for urethral repair. Urology. 1999;5:407–10.

    Article  Google Scholar 

  3. Schuller-Petrovic S, Wölkart G, Höfler G, Neuhold N, Freisinger F, Brunner F. Tissue-toxic effects of phosphatidylcholine/deoxycholate after subcutaneous injection for fat dissolution in rats and a human volunteer. Dermatol Surg. 2008;34:529–43.

    Article  CAS  PubMed  Google Scholar 

  4. Woods T, Gratzer PF. Effectiveness of three extraction techniques in the development of a decellularized bone-anterior cruciate ligament bone graft. Biomaterials. 2005;26:7339–49.

    Article  CAS  PubMed  Google Scholar 

  5. Guhathakurta S, Varghese S, Balasubramanian V, Agarwal R, Murthy BS, Veerappan S, et al. Technique to process xenogenic tissues for cardiovascular implantation: a preliminary report. Curr Sci. 2006;91:1068–73.

    CAS  Google Scholar 

  6. Paradkar MM, Singhal RS, Kulkarni PR. An approach to the detection of synthetic milk in dairy milk: detection of detergents. Int J Dairy Technol. 2000;5:92–5.

    Article  Google Scholar 

Download references

Acknowledgements

The authors wish to thank R. Balasundari and Sheerin Begam Nasser for help with decellularisation experiments, Dillip Kumar Bishi, Suneel Ralapalli, Kavitha Sankaranarayanan for scientific input and Pandian A. for technical assistance.

Author information

Authors and Affiliations

  1. Frontier Lifeline, Dr. K.M. Cherian Heart Foundation, R-30C Ambattur Industrial Estate Road, Mogappair, Chennai, 101, India

    Santosh Mathapati, Satish Galla, Kavitha Sankaranarayanan, Kotturathu Mammen Cherian & Soma Guhathakurta

  2. Department of Biotechnology, Indian Institute of Technology—Madras, Chennai, 36, India

    Santosh Mathapati & Rama Shanker Verma

Authors
  1. Santosh Mathapati
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Satish Galla
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kavitha Sankaranarayanan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rama Shanker Verma
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kotturathu Mammen Cherian
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Soma Guhathakurta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Soma Guhathakurta.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mathapati, S., Galla, S., Sankaranarayanan, K. et al. Qualitative and quantitative detection of sodium deoxycholic acid in decellularized tissue. Indian J Thorac Cardiovasc Surg 26, 129–131 (2010). https://doi.org/10.1007/s12055-010-0016-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12055-010-0016-x

Keywords

Search

Navigation