Abstract
Pineapple (Ananas comosus L. Merr) is a tropical plant with economic importance. We aimed to explore the antioxidase and transcriptome changes of pineapple in response to heat stress using cultivars ‘Xigua’ and ‘MD2′. The antioxidase activity of pineapple at different time points under high temperature (38 °C) was measured. The differentially expressed genes (DEGs) of the two pineapple varieties before and after high temperature treatment were screened and used to construct a protein to protein interaction (PPI) network. Subsequently, the expression of genes with high node degree in the PPI network was verified by qRT-PCR. The antioxidant enzyme activities varied in different cultivars with a time-dependent manner under high temperature. Totally 1214 DEGs was identified in ‘Xigua’ and 261 DEGs were identified in ‘MD2′. The enriched GO terms mainly included ‘response to hydrogen peroxide’ and ‘membrane part’. ‘nitrogen metabolism’ pathway and some secondary substance synthesis pathways were important KEGG pathways. A variety of heat shock proteins (HSPs) was identified to be related to the heat response of pineapple, and most of them showed a significantly high expression level in the high temperature group than that in the normal group. This study laid a certain foundation for the future research on the molecular mechanism of pineapple resistance to high temperature.
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The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
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Funding
This study is supported by the Special Research Project of Tropical Crops College of Yunnan Agricultural University (No. 2019RYZX001); Central Public-Interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences (No. 1630062020030); Key Research & Development Project of Shanxi Province (No. 201803D221011-5); and Scientific Research Fund of Yunnan Provincial Department of Education (No. 2020j0250).
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WZ devise the research and drafted the manuscript. ZM and SL acquired and analyzed the data. WY did a statistical analysis of the data. JM revised the manuscript for important intellectual content. All authors read and approved the final manuscript.
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Communicated by Yuan Qin.
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Supplementary file1 Figure S1. qRT-PCR verification the expression level between XGBL_T (38 °C) and XGBL_C (25 °C). * and ** represents p < 0.05 and p < 0.01, respectively. (TIF 863 KB)
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Supplementary file2 Figure S2. qRT-PCR verification the expression level between MD2_T (38 °C) and MD2_C (25 °C). * and ** represents p < 0.05 and p < 0.01, respectively. (TIF 878 KB)
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Supplementary file6 Table S4. The topological properties of protein nodes in the PPI network of 'MD2′ cultivar. (XLSX 59 KB)
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Supplementary file7 Table S5. The topological properties of protein nodes in the PPI network of 'Xigua' cultivar. (XLSX 227 KB)
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Supplementary file8 Table S6. The topological properties of protein nodes in the PPI network of 'Xigua' cultivar. (XLS 18 KB)
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Supplementary file9 Table S7. The topological properties of protein nodes in the PPI network of 'MD2′ cultivar. (XLS 16 KB)
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Zhao, W., Ma, Z., Liu, S. et al. Transcriptome Profiling Reveals Potential Genes and Pathways Supporting Ananas comosus L. Merr’s High Temperature Stress Tolerance. Tropical Plant Biol. 14, 132–142 (2021). https://doi.org/10.1007/s12042-021-09287-2
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DOI: https://doi.org/10.1007/s12042-021-09287-2