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SMRT sequencing of full-length transcriptome of birch-leaf pear (Pyrus betulifolia Bunge) under drought stress

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Abstract

Drought limits the pear yield and quality. The birch-leaf pear (Pyrus betulifolia Bunge) is one of the most frequently used pear rootstocks. Identifying genes involved in drought resistance of P. betulifolia would suggest candidate genes for molecular breeding. We used single-molecule long-read sequencing technology to investigate the transcriptome of birch-leaf pear under drought stress. As a result, 362,139 consensus reads were identified using six databases, among which 342,162 genes were functionally annotated. Further, we identified 7094 long noncoding RNAs. The sequencing data contained 9891 alternative splicing and 100,836 alternative polyadenylation events. We report here the full-length sequence of birch-leaf pear, which can be used for breeding enhanced varieties.

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Acknowledgements

This work was supported by Pear technical system of modern agricultural industry in Jiangsu—genetic and breeding (JATS[2018]269). Jiangsu Agricultural Science and Technology Innovation fund (Cx(19)2028), supported by Fearmarked Fund for Jiangsu Agricultural Industry Technology System (JATS [2020]390) and Jiangsu Agricultural Science and Technology Innovation Fund (SCx(19)3030).

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Author notes

  1. Qingsong Yang and Jinxing Wang contributed equally to this work.

Authors and Affiliations

  1. Institute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, Jiangsu, 200014, People’s Republic of China

    Qingsong Yang, Jinxing Wang, Zhonghua Wang, Xiaogang Li, Jialiang Kan & Jing Lin

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  1. Qingsong Yang
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  2. Jinxing Wang
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  3. Zhonghua Wang
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  4. Xiaogang Li
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  5. Jialiang Kan
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  6. Jing Lin
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Correspondence to Jing Lin.

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Corresponding editor: H. A. Ranganath

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Yang, Q., Wang, J., Wang, Z. et al. SMRT sequencing of full-length transcriptome of birch-leaf pear (Pyrus betulifolia Bunge) under drought stress. J Genet 100, 29 (2021). https://doi.org/10.1007/s12041-021-01272-8

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  • DOI: https://doi.org/10.1007/s12041-021-01272-8

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