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Cytological behaviour of floral organs and in silico characterization of differentially expressed transcript-derived fragments associated with ‘floral bud distortion’ in soybean

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Abstract

An attempt was made to understand the ‘floral bud distortion’ (FBD), an unexplored disorder prevailing in soybean. Cytological behaviour of floral reproductive organs and in silico characterization of differentially expressed transcript-derived fragments (TDFs) in symptomatic and asymptomatic soybean plants were carried out. Pollens in asymptomatic plants do not have defects in number, size, shape and function. However, in symptomatic plant, pollens were found nonviable, abnormal in shape and with reduced germination ability. Here, we employed a computational approach, exploring invaluable resources. The tissue-specific transcript profile of symptomatic and asymptomatic sources was compared to determine differentially expressed TDFs associated with FBD to improve its basic understanding. A total of 60 decamer primers produced 197 scorable amplicons, ranged 162–1130 bp, of which 171 were monomorphic and 26 were differentially regulated. Reproducible TDFs were sequenced and characterized for their homology analysis, annotation, protein–protein interaction, subcellular localization and their physical mapping. Homology-based annotation of TDFs in soybean revealed presence of two characterized and seven uncharacterized hits. Annotation of characterized sequences showed presence of genes, namely auxin response factor 9 (ARF9) and forkhead-associated (FHA) domain, which are directly involved in plant development through various pathways, such as hormonal regulation, plant morphology, embryogenesis and DNA repair.

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Acknowledgements

We sincerely thank the Department of Atomic Energy (DAE), Board of Research in Nuclear Sciences (BRNS), Government of India, Mumbai, India for providing financial support (grant no. 2013/37B/44/BRNS/1904).

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Authors and Affiliations

  1. Biotechnology Centre, Post Graduate Institute, Dr Panjabrao Deshmukh Krishi Vidyapeeth, Akola, 444 104, India

    PRASHANT B. KALE, PRAVIN V. JADHAV, RACHANA S. WAKEKAR, M. P. MOHARIL & R. G. DANI

  2. Nagarjuna Medicinal Garden, Post Graduate Institute, Dr Panjabrao Deshmukh Krishi Vidyapeeth, Akola, 444 104, India

    A. G. DESHMUKH

  3. Vasantrao Naik College of Agricultural Biotechnology, Dr Panjabrao Deshmukh Krishi Vidyapeeth, Yavatmal, 445 001, India

    M. S. DUDHARE

  4. Department of Agricultural Botany, Post Graduate Institute, Dr Panjabrao Deshmukh Krishi Vidyapeeth, Akola, 444 104, India

    R. S. NANDANWAR

  5. Department of Plant Pathology, Post Graduate Institute, Dr Panjabrao Deshmukh Krishi Vidyapeeth, Akola, 444 104, India

    S. S. MANE

  6. Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre (BARC), Trombay, Mumbai, 400 085, India

    J. G. MANJAYA

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  1. PRASHANT B. KALE
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  2. PRAVIN V. JADHAV
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Correspondence to PRAVIN V. JADHAV.

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Corresponding editor: Jitendra P. Khurana

Kale P. B., Jadhav P. V., Wakekar R. S., Moharil M. P., Deshmukh A. G., Dudhare M. S., Nandanwar R. S., Mane S. S., Manjaya J. G. and Dani R. G. 2016 Cytological behaviour of floral organs and in silico characterization of differentially expressed transcript-derived fragments associated with ‘floral bud distortion’ in soybean. J. Genet. 95, xx–xx

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KALE, P.B., JADHAV, P.V., WAKEKAR, R.S. et al. Cytological behaviour of floral organs and in silico characterization of differentially expressed transcript-derived fragments associated with ‘floral bud distortion’ in soybean. J Genet 95, 787–799 (2016). https://doi.org/10.1007/s12041-016-0693-3

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