Skip to main content

Advertisement

SpringerLink
Log in

Improved transfection of HUVEC and MEF cells using DNA complexes with magnetic nanoparticles in an oscillating field

  • Research Note
  • Published:
Journal of Genetics Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Dobson J. 2006 Gene therapy progress and prospects: magnetic nanoparticle-based gene delivery. Gene Ther. 13, 283–287.

    Article  PubMed  CAS  Google Scholar 

  • Dobson J. 2008 Remote control of cellular behaviour with magnetic nanoparticles. Nat. Nanotechnol. 3, 139–143.

    Article  PubMed  CAS  Google Scholar 

  • Fouriki A., Farrow N., Clements M. A. and Dobson J. 2010 Evaluation of the magnetic field requirements for magnetic nanoparticle-based gene transfection. NanoReviews 1, 5167.

    Google Scholar 

  • Jenkins S. I., Pickard M. R., Granger N. and Chari D. M. 2011 Magnetic nanoparticle-mediated gene transfer to oligodendrocyte precursor cell transplant populations is enhanced by magnetofection strategies. ACS Nano. 5, 6527–6538.

    Article  PubMed  CAS  Google Scholar 

  • Kamau S. W., Hassa P. O., Steitz B., Petri-Fink A., Hofmann H., Hofmann-Amtenbrink H. et al. 2006 Enhancement of the efficiency of non-viral gene delivery by application of pulsed magnetic field. Nucleic Acids Res. 34, e40.

    Article  Google Scholar 

  • Krötz F., Sohn H. Y., Glow T., Plank C. and Pohl U. 2003 Magnetofection potentiates gene delivery to cultured endothelial cells. J. Vasc. Res. 40, 425–434.

    Article  PubMed  Google Scholar 

  • Lee C. H., Kim J. H., Lee H. J., Jeon K., Lim H., Choi H. et al. 2011 The generation of iPS cells using non-viral magnetic nanoparticle based transfection. Biomaterials 32, 6683–6691.

    Article  PubMed  CAS  Google Scholar 

  • Liu Z., Zheng M., Meng F. and Zhong Z. 2011 Non-viral gene transfection in vitro using endosomal pH-sensitive reversibly hydrophobilized polyethylenimine. Biomaterials 32, 9109–9119.

    Article  PubMed  CAS  Google Scholar 

  • McBain S. C., Griesenbach U., Xenariou S., Keramane A., Batich C. D., Alton E. W. et al. 2008 Magnetic nanoparticles as gene delivery agents: enhanced transfection in the presence of oscillating magnet arrays. Nanotechnology 19, 405102.

    Article  PubMed  CAS  Google Scholar 

  • Muthana M., Scott S. D., Farrow N., Morrow F., Murdoch C., Grubb S. et al. 2008 A novel magnetic approach to enhance the efficacy of cell-based gene therapies. Gene Ther. 15, 902–910.

    Article  PubMed  CAS  Google Scholar 

  • Nagata D., Takahashi M., Sawai K., Tagami T., Usui T., Shimatsu A. et al. 2006 Molecular mechanism of the inhibitory effect of aldosterone on endothelial NO synthase activity. Hypertension 48, 165–171.

    Article  PubMed  CAS  Google Scholar 

  • Pickard M., and Chari D. 2010 Enhancement of magnetic nanoparticle-mediated gene transfer to astrocytes by ‘magnetofection’: effects of static and oscillating fields. Nanomedicine (Lond) 5, 217–232.

    Article  CAS  Google Scholar 

  • Plank C., Schillinger U., Scherer F., Bergemann C., Rémy J. S., Krötz F. et al. 2003 The magnetofection method: using magnetic force to enhance gene delivery. Biol. Chem. 384, 737–747.

    Article  PubMed  CAS  Google Scholar 

  • Seki T., Irie N., Nakamura K., Sakaue H., Ogawa W., Kasuga M. et al. 2006 Fused protein of deltaPKC activation loop and PDK1-interacting fragment (deltaAL-PIF) functions as a pseudosubstrate and an inhibitory molecule for PDK1 when expressed in cells. Genes Cells 11, 1051–1070.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. nanoTherics Limited, Med IC4, Keele University Science and Business Park, Keele, Staffordshire, ST5 5NL, UK

    JENSON LIM

  2. J. Crayton Pruitt Family Department of Biomedical Engineering and Department of Materials Science and Engineering, University of Florida, Gainesville, FL 32611, USA

    JON DOBSON

  3. Institute for Science and Technology in Medicine, Keele University, Stoke-on-Trent, ST4 7QB, UK

    JON DOBSON

Authors
  1. JENSON LIM
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. JON DOBSON
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to JENSON LIM.

Additional information

[Lim J. and Dobson J. 2012 Improved transfection of HUVEC and MEF cells using DNA complexes with magnetic nanoparticles in an oscillating field. J. Genet. 91, xx–xx]

Rights and permissions

Reprints and permissions

About this article

Cite this article

LIM, J., DOBSON, J. Improved transfection of HUVEC and MEF cells using DNA complexes with magnetic nanoparticles in an oscillating field. J Genet 91, 223–227 (2012). https://doi.org/10.1007/s12041-012-0164-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12041-012-0164-4

Keywords

Search

Navigation