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Phosphorylation mapping of Laminin β1-chain: Kinases in association with active sites

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Abstract

Laminins are a major constituent of the extracellular matrix (ECM). Laminin-111, the most extensively studied laminin isoform, consists of the α1, the β1 and the γ1 chain, and is involved in many cellular processes, like adhesion, migration and differentiation. Given the regulatory role of phosphorylation in protein function, it is important to identify the phosphorylation sites of human laminin β1-chain sequence (LAMB1). Therefore, we computationally predicted all possible phosphorylation sites in LAMB1. For the first time, we identified the possibly responsible kinases for already in vitro experimentally observed phosphorylated residues in LAMB1. All known functional (active) sites of LAMB1, were recorded after an extensive literature search and combined with the experimentally observed and our predicted phosphorylated residues. This generated a detailed phosphorylation map of LAMB1. Five kinases (PKA, PKC, CKII, CKI and GPCR1) were indicated important, while the role of PKA, PKC and CKII, kinases known for ecto-phosphorylation activity, was highlighted. The activity of PKA and PKC was associated with the active site RIQNLLKITNLRIKFVKLHTLGDNLLDS. Also, predicted phosphorylations inside two amyloidogenic (DSITKYFQMSLE, VILQHSAADIAR) and two anti-cancerous (YIGSR and PDSGR) sites suggested a possible role in the development of the corresponding diseases.

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Abbreviations

AD:

Alzheimer disease

Akt:

Ak strain transforming (alternative name, protein kinase B)

ALK:

anaplastic lymphoma kinase

AMPK:

5′ adenosine monophosphate-activated protein kinase

APP:

β–amyloid precursor protein

ATM:

ataxia telangiectasia mutated

Aβ:

amyloid-β peptides

BARK1:

beta-adrenergic receptor kinase

CaMKII:

calmodulin-dependent kinase II

CaMKIV:

calmodulin-dependent kinase IV

CDK5:

cyclin-dependent kinase 5

Chk1:

checkpoint kinase 1

CKI:

casein kinase I

CKII:

casein kinase II

CLK1:

dual specificity protein kinase CLK1 (alternative name, CDC-like kinase 1)

COB:

cobblestone brain malformation

DNA-PK:

deoxyribonucleic acid protein kinase

ECM:

extracellular matrix

EGFR:

epidermal growth factor receptor

ERK1:

extracellular signal-regulated kinase 1

ERK2:

extracellular signal-regulated kinase 2

GPCR1:

G-coupled protein receptor 1

GSK-3:

glycogen synthase kinase-3

H1K:

growth-associated histone H1 kinase

HTP:

high-throughput (option in PhosphositePlus®)

JAK2:

janus kinase 2

La/SSB:

Sjögren syndrome type B antigen (alternative name, lupus La protein)

MAPKAPK1:

mitogen-activated protein (MAP) kinase-activated protein kinase 1

MAPKAPK2:

mitogen-activated protein (MAP) kinase-activated protein kinase 2

MMP-2:

matrix metalloproteinase-2

ONH:

optic nerve hypoplasia

p70S6K:

ribosomal protein S6 kinase

PAK2:

P21 (RAC1) activated kinase 2

PDGFRα:

platelet-derived growth factor receptor alpha

PDK:

pyruvate dehydrogenase kinase

PKA:

protein kinase A

PKC:

protein kinase C

PLK1:

polo-like kinase 1

PO3 2− :

phosphoryl group

PO4 3− :

phosphate group

PTKs:

protein tyrosine kinases

PTM:

post-translational modification

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Acknowledgements

We thank Papavramidis Theodosis for his assistance and constructive advice in preparing figure 2.

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    1. Laboratory of Biological Chemistry, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, University Campus, 54124, Thessaloniki, Greece

      Kleio-Maria Verrou, Panagiota Angeliki Galliou, Maria Papaioannou & Georgios Koliakos

    2. School of Medicine, University of Crete, Voutes University Campus, 70013, Heraklion, Crete, Greece

      Kleio-Maria Verrou

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    1. Kleio-Maria Verrou
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    Correspondence to Georgios Koliakos.

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    Communicated by Kundan Sengupta.

    Corresponding editor: K undan S engupta

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    Verrou, KM., Galliou, P.A., Papaioannou, M. et al. Phosphorylation mapping of Laminin β1-chain: Kinases in association with active sites. J Biosci 44, 55 (2019). https://doi.org/10.1007/s12038-019-9871-9

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