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Extra-oviductal expression of oviductal glycoprotein 1 in mouse: Detection in testis, epididymis and ovary

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Abstract

Oviductal glycoprotein 1 (OVGP1), also called oviductin, is an oviduct-specific protein and is suggested to play a role in fertilization. Traditionally, Ovgp1 has been shown to be exclusively expressed by the oviduct; however, recent studies have demonstrated its expression in some cancers. This observation led us to hypothesize that Ovgp1 might have some extra-oviductal expression. In the current study, we evaluated the mRNA and protein expression of Ovgp1 in normal reproductive tissues of male and female mice. For the first time, we demonstrate that beyond the oviduct, Ovgp1 mRNA is expressed in the testis, epididymis and ovary, but not in the uterus, cervix, vagina, breast, seminal vesicles and prostate gland. In the testis, Ovgp1 mRNA was localized in the cells at the base of seminiferous tubules (most likely, Sertoli cells), while the protein was detected in the round and elongating spermatids. In the epididymis, Ovgp1 transcripts were localized in epididymal epithelium of the caput but not the corpus and cauda; OVGP1 protein was, however, not detected in any of the segments but was present in the epididymal sperm. In the ovary, Ovgp1 transcripts and protein were detected in the surface epithelium, granulosa cells of the preantral and the antral follicles and corpus luteum. In both, the ovary and oviduct, the expression of Ovgp1 was found to be higher at estrus stage than at diestrus stage. To the best of our knowledge, this is the first study demonstrating the extra-oviductal expression of Ovgp1. Our data suggests that, beyond fertilization, Ovgp1 might have specific roles in gonadal physiology.

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Acknowledgements

The study was supported by NMIMS University. We thank Dr Aparna Khanna, Dean, SDSOS, NMIMS University, and Dr Smita Mahale, Director, NIRRH, for providing necessary support and facilities. We sincerely thank Dr Nafisa Balasinor, NIRRH, for providing the microtome facility. We acknowledge Mr Suryakant for his technical assistance with sectioning.

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Authors and Affiliations

  1. Department of Biological Sciences, Sunandan Divatia School of Science, NMIMS University, Vile Parle (West), Mumbai, 400 056, India

    Saniya Laheri & Purvi Bhatt

  2. Molecular and Cellular Biology Laboratory, National Institute for Research in Reproductive Health (ICMR), JM Street, Parel, Mumbai, 400 012, India

    Deepak Modi

Authors
  1. Saniya Laheri
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  2. Deepak Modi
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  3. Purvi Bhatt
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Correspondence to Purvi Bhatt.

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Corresponding editor: S Shivaji

[Laheri S, Modi D and Bhatt P 2017 Extra-oviductal expression of oviductal glycoprotein 1 in mouse: Detection in testis, epididymis and ovary. J. Biosci.]

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Laheri, S., Modi, D. & Bhatt, P. Extra-oviductal expression of oviductal glycoprotein 1 in mouse: Detection in testis, epididymis and ovary. J Biosci 42, 69–80 (2017). https://doi.org/10.1007/s12038-016-9657-2

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  • DOI: https://doi.org/10.1007/s12038-016-9657-2

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