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Photodynamic effect of light-emitting diode light on cell growth inhibition induced by methylene blue

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Abstract

The aim of this study was to propose the use of red light-emitting diode (LED) as an alternative light source for methylene blue (MB) photosensitizing effect in photodynamic therapy (PDT). Its effectiveness was tested against Staphylococcus aureus (ATCC 26923), Escherichia coli (ATCC 26922), Candida albicans (ATCC 90028) and Artemia salina. The maximum absorption of the LED lamps was at a wavelength of 663 nm, at intensities of 2, 4, 6 and 12 J.cm−2 for 10, 20, 30 and 60 min of exposure, respectively. Assays with and without LED exposure were carried out in plates containing MB at concentrations of 7 to 140.8 μM for microorganisms and 13.35 to 668.5 μM for microorganisms or microcrustaceans. The LED exposure induced more than 93.05%, 93.7% and 93.33% of growth inhibition for concentrations of 42.2 μM for S. aureus (D-value=12.05 min) and 35.2 μM for E. coli (D-value=11.51 min) and C. albicans (D-value=12.18 min), respectively after 20 min of exposure. LED exposure for 1 h increased the cytotoxic effect of MB against A. salina from 27% to 75%. Red LED is a promising light device for PDT that can effectively inhibit bacteria, yeast and microcrustacean growth.

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Abbreviations

CFU:

colony-forming unit

IC50:

inhibition of 50% growth

LED:

light-emitting diode

MB:

methylene blue

MHB:

Mullen-Hinton broth

%T:

percentage of transmittance

PDT:

photodynamic therapy

References

  • Borissevitch I E and Gandini S C M 1998 Photophysical studies of excited-state characteristics of meso-tetrakis (4-N-methyl-pyridiniumyl) porphyrin bound to DNA; J. Photochem. Photobiol. B: Biol. 43 112–120

    Article  CAS  Google Scholar 

  • Calzavara-Pinton P G, Venturini M and Sala R 2005 A comprehensive overview of photodynamic therapy in the treatment of superficial fungal infections of the skin; J. Photochem. Photobiol. B: Biol. 78 1–6

    Article  CAS  Google Scholar 

  • Carballo J L, Hernández-Ida Z L, Pérez P and García-Grávalos M D 2002 A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products; BMC Biotechnology 472 2–17

    Google Scholar 

  • Hostettmann K 1991 Assays for bioactivity; in Methods in plant biochemistry (eds) P M Dey and J B Harbarne (London: Academic Press) pp 6–9

    Google Scholar 

  • Kinghorn A D, Harjes K K and Doorenbos N J 1977 Screening procedure for phorbol esters using brine shrimp (Artemia salina) larvae; J. Pharm. Sci. 66 1362–1363

    Article  CAS  Google Scholar 

  • Lambrechts S A G, Aalders M C G and Van Marle J 2005 Mechanistic study of the photodynamic inactivation of Candida albicans by a cationic porphyrin; Antimicrob. Agents Chemother. 49 2026–2034

    Article  CAS  Google Scholar 

  • Levy J G 1995 Photodynamic therapy; Trends Biotechnol. 13 14–18

    Article  CAS  Google Scholar 

  • Livermore D M 1990 Antibiotic replace and transport by bacteria; Scand. J. Infect. Dis.-Suppl. 74 15–22

    CAS  PubMed  Google Scholar 

  • Machado A E H 2000 Photodynamic therapy: principles, potential of application and perspectives; Quimica Nova 23 237–243

    Article  Google Scholar 

  • Mang T S 2004 Lasers and light sources for PDT: past, present and future; Photodiagnosis Photodynamic Therapy 1 43–48

    Article  Google Scholar 

  • McLaughlin J L, Rogers L L and Anderson J E 1998 The use of biological assays to evaluate botanicals; Drug Inf. J. 32 513–524

    Article  Google Scholar 

  • Panda K and Chakraborty A K 1997 Studies on the interaction of bacterial lipopolysaccharide with cationic dyes by absorbance and fluorescence spectroscopy; J. Photochem. Photobiol. A: Chem. 111 157–162

    Article  CAS  Google Scholar 

  • Phoenix D A, Sayed Z, Hussain S, Harris F and Wainwright M 2003 The phototoxicity of phenothiazinium derivatives against Escherichia coli and Staphylococcus aureus; FEMS Immunol. Med. Microbiol. 39 17–22

    Article  CAS  Google Scholar 

  • Souza S C, Junqueira J C, Balducci I, Koga-Ito C Y, Munin E and Jorge A O C 2006 Photosensitization of different Candida species by low power laser light; J. Photochem. Photobiol. B: Biol. 83 34–38

    Article  Google Scholar 

  • Sternberg E D and Dolphin D 1996 Pyrrolic photosensitizers; Curr. Medicinal Chem. 3 293–324

    Google Scholar 

  • Sternberg E D, Dolphin D and Bruckner C 1998 Porphyrin-based photosensitizers for use in photodynamic therapy; Tetrahedron 54 4151–4202

    Article  CAS  Google Scholar 

  • Sud I J and Feingold D S 1981 Heterogeneity of action mechanisms among antimycotic imidazoles; Antimicrob. Agents Chemother. 20 71–74

    Article  CAS  Google Scholar 

  • Tardivo J P, Giglio A D, Paschoal L H C, Ito A S and Baptista M S 2004 Treatment of melanoma lesions using methylene blue and RL50 light source; Photodiagnosis Photodynamic Therapy 1 345–346

    Article  CAS  Google Scholar 

  • Tardivo J P, Giglio A D, Oliveira C S, Gabrielli D S, Junqueira H C, Tada D B, Severino D, Turchiello R F and Baptista M S 2005 Methylene blue in photodynamic therapy: from basic mechanisms to clinical applications; Photodiagnosis Photodynamic Therapy 2 1–17

    Article  Google Scholar 

  • Usacheva M N, Teichert M C and Biel M A 2001 Comparison of the methylene blue and toluidine blue photobactericidal efficacy against Gram-positive and Gram-negative microorganisms; Lasers Surg. Med. 29 165–173

    Article  CAS  Google Scholar 

  • Usacheva M N, Teichert M C and Biel M A 2003 The role of the methylene blue and toluidine blue monomers and dimers in the photoinactivation of bacteria; J. Photochem. Photobiol. B: Biol. 71 87–98

    Article  CAS  Google Scholar 

  • Wainwright M 1998 Photodynamic antimicrobial chemotherapy (PACT); J. Antimicrob. Chemother. 42 13–28

    Article  CAS  Google Scholar 

  • Wainwright M and Crossley K B 2002 Methylene blue — a therapeutic dye for all seasons; J. Chemother. 14 431–443

    Article  CAS  Google Scholar 

  • Wainwright M, Phoenix D A, Marland J, Wareing D R A and Bolton F J 1997 A study of photobactericidal activity in the phenothiazinium series; FEMS Immunol. Med. Microbiol. 19 75–80

    Article  CAS  Google Scholar 

  • Weishaupt K R, Gomer C J and Dougherty T J 1976 Identification of singlet oxygen as the cytotoxic agent in photoinactivation of a murine tumor; Cancer Res. 36 2326–2329

    CAS  PubMed  Google Scholar 

  • Wilson M and Mia N 1993 Sensitization of Candida albicans to killing by low-power laser light; J. Oral Pathol. Med. 22 354–357

    Article  CAS  Google Scholar 

  • Zeina B, Greenman J, Purcell W M and Das B 2001 Killing of cutaneous microbial species by photodynamic therapy; Br. J. Dermatol. 144 274–278

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Chemistry Department, State University of Maringá, Paraná, Brazil

    Lílian S. Peloi, Rafael R. S. Soares, Caruos E. G. Biondo, Vagner R. Souza & Noboru Hioka

  2. Department of Pharmacy and Pharmacology, State University of Maringá, Paraná, Brazil

    Elza Kimura

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  1. Lílian S. Peloi
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  2. Rafael R. S. Soares
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  3. Caruos E. G. Biondo
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Correspondence to Elza Kimura.

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Peloi, L.S., Soares, R.R.S., Biondo, C.E.G. et al. Photodynamic effect of light-emitting diode light on cell growth inhibition induced by methylene blue. J Biosci 33, 231–237 (2008). https://doi.org/10.1007/s12038-008-0040-9

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  • DOI: https://doi.org/10.1007/s12038-008-0040-9

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