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Investigation of Relationship Between Small Noncoding RNA (sncRNA) Expression Levels and Serum Iron, Copper, and Zinc Levels in Clinical Diagnosed Multiple Sclerosis Patients

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Abstract

In our study, we aimed to investigate the relationship between microRNA (miRNA) expression levels and serum iron (Fe), copper (Cu), and zinc (Zn) levels in Multiple sclerosis (MS) patients. Total RNA was isolated from peripheral venous blood containing ethylenediaminetetraacetic acid (EDTA) of MS patients and controls. Total RNA was labeled with Cy3-CTP fluorescent dye. Hybridization of samples was performed on microarray slides and arrays were scanned. Data argument and bioinformatics analysis were performed. Atomic absorption spectrophotometer method was used to measure serum Fe, Cu, and Zn levels. In our study, in bioinformatics analysis, although differently expressed miRNAs were not detected between 16 MS patients and 16 controls, hsa-miR-744-5p upregulation was detected between 4 MS patients and 4 controls. This may be stem from the patient group consisting of MS patients who have never had an attack for 1 year. Serum iron levels were detected significantly higher in the 16 MS patients compared to the 16 controls. This may be stem from the increase in iron accumulation based on inflammation in MS disease. According to the findings in our study, hsa-miR-744-5p upregulation has been determined as an early diagnostic biomarker for the development together of insulin resistance, diabetes mellitus associated with insulin signaling, and Alzheimer’s diseases. Therefore, hsa-miR-744-5p is recommended as an important biomarker for the development together of diabetes mellitus, Alzheimer’s disease, and MS disease. In addition, increased serum Fe levels may be suggested as an important biomarker for neurodegenerative diseases such as Parkinson’s disease, Alzheimer’s disease, and MS disease.

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Data Availability

Data sharing is not applicable to this article as no datasets were generated or analyzed during the current study.

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Acknowledgements

Our study was carried out in the Department of Biophysics, Department of Medical Genetics, and Department of Neurology at Trakya University.

Funding

This study was supported by the Coordinatorship of Scientific Research Projects of Trakya University with 2017/68 project number.

Author information

Authors and Affiliations

  1. Department of Biophysics, Faculty of Medicine, Trakya University, Edirne, 22030, Turkey

    Arzu Ay, Tevfik Gulyasar & Tammam Sipahi

  2. Department of Biophysics, Faculty of Medicine, Haliç University, Istanbul, 34060, Turkey

    Nevra Alkanli

  3. Department of Medical Genetics, Faculty of Medicine, Trakya University, Edirne, 22030, Turkey

    Engin Atli & Hakan Gurkan

  4. Department of Neurology, Faculty of Medicine, Trakya University, Edirne, 22030, Turkey

    Sibel Guler

  5. Department of Biostatistics and Medical Informatics, Faculty of Medicine, Trakya University, Edirne, 22030, Turkey

    Necdet Sut

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  1. Arzu Ay
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Contributions

AA and NA designed the experiments. AA, NA, EA, HG, TG, and TS performed the experiments. AA, NA, EA, and HG analyzed the data. Samples collection was performed by AA and SG. Statistical analyses were performed by NS. AA and NA wrote the paper. All authors read and approved the final manuscript. The corresponding author attests that all listed authors meet the authorship criteria and that no other authors meeting the criteria have been omitted.

Corresponding author

Correspondence to Arzu Ay.

Ethics declarations

Ethics Approval

The approval of the ethics committee was obtained from the Trakya University Faculty of Medicine Non-Invasive Ethics Committee (Protocol code: TÜTF-BAEK 2017/11).

Informed Consent

Signed informed consent forms were collected from each of the MS patients and healthy controls.

Consent for Publication

The author, Arzu Ay, Nevra Alkanli, Engin Atli, Hakan Gurkan, Tevfik Gulyasar, Sibel Guler, Tammam Sipahi, Necdet Sut have read and approved the final manuscript for submission. We confirm the tables and figures are original for this article.

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The authors declare no competing interests.

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Ay, A., Alkanli, N., Atli, E. et al. Investigation of Relationship Between Small Noncoding RNA (sncRNA) Expression Levels and Serum Iron, Copper, and Zinc Levels in Clinical Diagnosed Multiple Sclerosis Patients. Mol Neurobiol 60, 875–883 (2023). https://doi.org/10.1007/s12035-022-03135-4

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