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Genetic Characterization of Escherichia coli O157:H7 Strains Isolated from the One-Humped Camel (Camelus dromedarius) by Using Microarray DNA Technology

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Abstract

From the Camelidae family members, several serotypes of Escherichia coli (E. coli) have recently been isolated from diarrhoeic and non-diarrhoeic faecal samples. To date Shiga toxin-producing E. coli (STEC) strains have never been typed in one-humped camel (Camelus dromedarius). In the present study, two E. coli O157:H7 strains isolated from sick dromedaries were investigated. Virulence gene profiles were determined using a custom E. coli virulence DNA microarray, composed of 70-mer oligonucleotide probes targeting 264 virulence or related genes of known E. coli pathotypes. Both strains displayed positive hybridization signals for the Locus of enterocyte effacement (LEE) gene probes (ler, eae, espA, espB, tir genes), two Shiga toxin probes (stx1 and stx2), the O157 O-antigen specific probe, various virulence plasmid (pO157) probes like katP in addition to other accessory virulence genes characterized in STEC.

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Acknowledgment

We thank Dr. Armando Giovannini of Istituto G. Caporale, Epidemiology unit, for his suggestions.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Gharib Avenue, Azadi Street, 14155-6453, Tehran, Iran

    Taghi Zahraei Salehi & Iradj Ashrafi Tamai

  2. Istituto “G. Caporale”, Campo Boario, 64100, Teramo, Italy

    Alfreda Tonelli, Pietro Badagliacca & Rossella Lelli

  3. Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount, Montreal, QC, H4P 2R2, Canada

    Alberto Mazza & Luke Masson

  4. Department of Microbiology, Faculty of Veterinary Medicine, University of Semnan, Shahmirzad, 35718-95589, Semnan, Iran

    Hamid Staji & Reza Jamshidi

  5. Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, 3200 rue Sicotte, Saint-Hyacinthe, Montreal, QC, J2S 7C6, Canada

    Josée Harel

Authors
  1. Taghi Zahraei Salehi
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  2. Alfreda Tonelli
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  3. Alberto Mazza
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  4. Hamid Staji
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  5. Pietro Badagliacca
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  6. Iradj Ashrafi Tamai
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  7. Reza Jamshidi
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  8. Josée Harel
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  9. Rossella Lelli
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  10. Luke Masson
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Correspondence to Pietro Badagliacca.

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Salehi, T.Z., Tonelli, A., Mazza, A. et al. Genetic Characterization of Escherichia coli O157:H7 Strains Isolated from the One-Humped Camel (Camelus dromedarius) by Using Microarray DNA Technology. Mol Biotechnol 51, 283–288 (2012). https://doi.org/10.1007/s12033-011-9466-7

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  • DOI: https://doi.org/10.1007/s12033-011-9466-7

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