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Prays oleae Midgut Putative Receptor of Bacillus thuringiensis Vegetative Insecticidal Protein Vip3LB Differs from that of Cry1Ac Toxin

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Abstract

Vegetative insecticidal protein (Vip) is a class of insecticidal proteins produced by many Bacillus thuringiensis strains during their vegetative growth stage. The vip3LB gene of B. thuringiensis strain BUPM95, which encodes a protein active against the Lepidoptera olive tree pathogenic insect Prays oleae, was cloned into pET-14b vector and overexpressed in Escherichia coli. The expressed Vip3LB protein, found in the E. coli cytoplasmic fraction, was purified and used to produce anti-Vip3LB antibodies. Using the midgut extract of P. oleae, the purified Vip3LB bound to a 65-kDa protein, whereas Cry1Ac toxin bound to a 210-kDa midgut putative receptor. This result justifies the importance of the biological pest control agent Vip3LB that could be used as another alternative particularly in case of resistance to Cry toxins.

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Abbreviations

B :

Bacillus

P :

Prays

IgG:

Immunoglobulin G

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Acknowledgments

This work was supported by grants from the Ministry of Higher Education, Scientific Research and Technology. We thank the Institut de l’olivier de Sfax, Tunisia for providing us with P. oleae larvae. We wish to thank Pr. Jamil Jaoua, Head of the English Unit at the Sfax Faculty of Science for having proofread this paper.

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Authors and Affiliations

  1. Laboratory of Biopesticides, Centre of Biotechnology of Sfax, P.O. Box 1177, 3018, Sfax, Tunisia

    Lobna Abdelkefi-Mesrati, Souad Rouis, Sameh Sellami & Samir Jaoua

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  1. Lobna Abdelkefi-Mesrati
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  2. Souad Rouis
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  3. Sameh Sellami
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  4. Samir Jaoua
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Correspondence to Samir Jaoua.

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Abdelkefi-Mesrati, L., Rouis, S., Sellami, S. et al. Prays oleae Midgut Putative Receptor of Bacillus thuringiensis Vegetative Insecticidal Protein Vip3LB Differs from that of Cry1Ac Toxin. Mol Biotechnol 43, 15–19 (2009). https://doi.org/10.1007/s12033-009-9178-4

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