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A Simple Silica-based Method for Metagenomic DNA Extraction from Soil and Sediments

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Abstract

A new method is described for extraction of metagenomic DNA from soil and sediments which is based on DNA adsorption to silica without the use of phenol, ethanol precipitation or a cesium chloride gradient. High-quality DNA was obtained, and PCR inhibition was overcome by adding bovine serum albumin and adjusting magnesium concentration. By using PCR-DGGE with Firmicutes and lactic acid bacteria-specific primers the extracted metagenomic DNA was shown to contain a mixture of bacterial genomes. This method can be used for screening bacterial diversity in soil and sediment samples.

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Acknowledgments

This research was supported by the “Fondo Sectorial de Investigación Ambiental” from Mexico, Project FOSEMARNAT-2004-01-280.

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Authors and Affiliations

  1. Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Av. Juárez No. 421, Ciudad Industrial, Apdo. Postal 1226-A, Mérida, Yucatán, Mexico

    R. Rojas-Herrera & M. Zamudio-Maya

  2. Centro de Biotecnología Genómica del Instituto Politécnico Nacional, Reynosa, Tamaulipas, Mexico

    J. Narváez-Zapata

  3. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Guadalajara , Jalisco, Mexico

    M. E. Mena-Martínez

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  1. R. Rojas-Herrera
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  2. J. Narváez-Zapata
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  3. M. Zamudio-Maya
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  4. M. E. Mena-Martínez
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Correspondence to R. Rojas-Herrera.

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Rojas-Herrera, R., Narváez-Zapata, J., Zamudio-Maya, M. et al. A Simple Silica-based Method for Metagenomic DNA Extraction from Soil and Sediments. Mol Biotechnol 40, 13–17 (2008). https://doi.org/10.1007/s12033-008-9061-8

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  • DOI: https://doi.org/10.1007/s12033-008-9061-8

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