Abstract
A new method is described for extraction of metagenomic DNA from soil and sediments which is based on DNA adsorption to silica without the use of phenol, ethanol precipitation or a cesium chloride gradient. High-quality DNA was obtained, and PCR inhibition was overcome by adding bovine serum albumin and adjusting magnesium concentration. By using PCR-DGGE with Firmicutes and lactic acid bacteria-specific primers the extracted metagenomic DNA was shown to contain a mixture of bacterial genomes. This method can be used for screening bacterial diversity in soil and sediment samples.
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This research was supported by the “Fondo Sectorial de Investigación Ambiental” from Mexico, Project FOSEMARNAT-2004-01-280.
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Rojas-Herrera, R., Narváez-Zapata, J., Zamudio-Maya, M. et al. A Simple Silica-based Method for Metagenomic DNA Extraction from Soil and Sediments. Mol Biotechnol 40, 13–17 (2008). https://doi.org/10.1007/s12033-008-9061-8
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DOI: https://doi.org/10.1007/s12033-008-9061-8