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The effect of dual-frequency sonication in the presence of thalidomide angiogenesis inhibitor and nanomicelles containing doxorubicin on inhibiting the growth and angiogenesis of breast adenocarcinoma in vivo

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Abstract

This study aimed to evaluate the effect of dual-frequency sonication in the presence of thalidomide angiogenesis inhibitor and nanomicelles containing doxorubicin on inhibiting the growth and angiogenesis of breast adenocarcinoma in BALB/c female mice. Sixty mice carrying the tumor were divided into 12 groups: (A) control, (B) 28 kHz and 3 MHz sonication, (C) thalidomide, (D) thalidomide and 28 kHz, (E) thalidomide and 3 MHz, (F) thalidomide and dual-frequency sonication, (G) doxorubicin, (H) nanomicelles containing doxorubicin, (I) nanomicelles containing doxorubicin and dual-frequency sonication, (J) thalidomide and doxorubicin, (K) thalidomide and nanomicelles containing doxorubicin, and (L) thalidomide and nanomicelles containing doxorubicin and dual-frequency sonication. The delay in the tumor growth and angiogenesis percent were extracted. Pathological and immunohistochemical studies were performed to confirm the treatment. The findings of tumor growth retardation parameters and animal survival were significantly different in group L from all groups (P < 0.05). The highest rate of inhibition was in group L with a 46% inhibition. In group L, 100% of the animals survived until day 49. In groups F, C, G, B, and A, all the animals survived 45, 42, 39, 32, and 30 days, respectively. Pathological results showed a decrease in tumor grade in groups K and L. Histopathological results demonstrate a decrease in group L angiogenesis compared to group C. These findings were consistent with the results of color Doppler ultrasound imaging. Dual-frequency sonication in the presence of thalidomide and doxorubicin-containing nanomicelles inhibits tumor growth and angiogenesis.

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All relevant data are included within the paper and supporting information files. Please contact the corresponding author for material availability.

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Acknowledgements

This study was approved by the Faculty of Medical Sciences, Tarbiat Modares University.

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Authors and Affiliations

  1. Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Zohreh Goleh & Manijhe Mokhtari-Dizaji

  2. Department of Pharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

    Tayebeh Toliyat

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  1. Zohreh Goleh
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  2. Manijhe Mokhtari-Dizaji
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  3. Tayebeh Toliyat
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Contributions

All authors contributed to the study’s conception and design. MMD, as the corresponding author, was the chief investigator. ZG and MMD participated in the design of the work and data and image acquisition; they conducted the statistical analysis, drafted the manuscript, and reviewed and revised this paper. TT participated in data acquisition and design and preparation of nanomicelles. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Manijhe Mokhtari-Dizaji.

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The animal experiments followed the guidelines of the Laboratory Animal Ethical Committee of the Faculty of Medical Sciences, Tarbiat Modares University (IR.TMU.REC.1396.33).

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Goleh, Z., Mokhtari-Dizaji, M. & Toliyat, T. The effect of dual-frequency sonication in the presence of thalidomide angiogenesis inhibitor and nanomicelles containing doxorubicin on inhibiting the growth and angiogenesis of breast adenocarcinoma in vivo. Med Oncol 40, 20 (2023). https://doi.org/10.1007/s12032-022-01898-3

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