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Trillin prevents proliferation and induces apoptosis through inhibiting STAT3 nuclear translocation in hepatoma carcinoma cells

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Abstract

Trillin is a constituent of total Trillium Tschonoskii Maxim (TTM), which is extracted from TTM and displayed anti-tumor effect in many tumor cell lines. However, the anti-tumor mechanism of trillin is still unclear. This study demonstrated that trillin could dramatically inhibit hepatoma carcinoma cell proliferation, induce apoptosis and decrease migration and invasion through suppressing phosphorylated STAT3 translocated to nucleus. Trillin could down-regulate Bcl-2 and Survivin, up-regulate cleaved PRAP, leading to dramatically apoptosis; trillin could also down-regulate MMP1, MMP2, MucI and VEGF, which displayed an inhibition effect on hepatocellular tumor cells invasion and development. The results of this study indicated the potential utility of trillin as a STAT3 inhibitor for the treatment of cancers.

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Acknowledgements

This work was supported by the Grant of National Natural Science Foundation of China (Grant No. 81660718); Foundation of Health and Family Planning Commission of Hubei (Grant No. WJ2017M175); the Natural Science Foundation Hubei Province of China (2012FFB04903).

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Author notes

  1. Guangjie Zhan and Jun Hu have contributed equally to this work.

Authors and Affiliations

  1. Medical School of Hubei MinZu University, Enshi, 445000, Hubei, People’s Republic of China

    Guangjie Zhan & Benjian Xiao

  2. New Medicine Innovation and Development Institute, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, College of Medicine, Wuhan University of Science and Technology, Wuhan, 430065, Hubei, People’s Republic of China

    Lili Lu

  3. Demonstration Center for Experimental Basic Medicine Education of Wuhan University, Wuhan, 430071, Hubei, People’s Republic of China

    Jun Hu, Zixian Yang & Guohua Yang

  4. Science and Technology College of Hubei MinZu University, Enshi, 445000, Hubei, People’s Republic of China

    Xianli Wang

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  1. Guangjie Zhan
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Contributions

GZ, BX and XW participated in the experiments and data collection. JH drafted the manuscript. ZY did the statistical analysis of data. GY participated in the design of the study and the general supervision of the research group. LL made strict revision of this manuscript and drafted parts of it.

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Correspondence to Guohua Yang or Lili Lu.

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Zhan, G., Hu, J., Xiao, B. et al. Trillin prevents proliferation and induces apoptosis through inhibiting STAT3 nuclear translocation in hepatoma carcinoma cells. Med Oncol 37, 44 (2020). https://doi.org/10.1007/s12032-020-01369-7

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  • DOI: https://doi.org/10.1007/s12032-020-01369-7

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