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Epigallocatechin-3-gallate induces apoptosis and cell cycle arrest in HTLV-1-positive and -negative leukemia cells

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Abstract

The objective of this study is to evaluate the efficacy of epigallocatechin gallate against ATL cells. The anti-proliferative and pro-apoptotic effects of EGCG were evaluated in HTLV-1-positive and -negative cells. EGCG exhibited a marked decrease in proliferation of ATL cells at 96 h of treatment. The results indicated that TGF-α was down-regulated whereas levels of TGF-β2 increased. Cell cycle distribution analysis revealed an increase in cells in the pre-G1 phase which was confirmed by ELISA. The results on proteins showed an up-regulation of p53, Bax and p21 protein levels while the levels of Bcl-2α were down-regulated.

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Acknowledgements

Our thanks to the Science Core Laboratory at the American University of Beirut and to Mrs Rania El-osta and Mr. Joe El Khoury for help with the typing and figure editing of this manuscript.

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Authors and Affiliations

  1. Biology Department, American University of Beirut, Beirut, 11-0236, Lebanon

    S. Harakeh, K. Abu-El-Ardat & M. Diab-Assaf

  2. Dr. Rath Research Insitute, Santa Clara, CA, USA

    A. Niedzwiecki & M. Rath

  3. Human Morphology Department, American University of Beirut, Beirut, 11-0236, Lebanon

    M. El-Sabban

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  1. S. Harakeh
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  2. K. Abu-El-Ardat
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  3. M. Diab-Assaf
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  6. M. Rath
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Correspondence to S. Harakeh.

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Harakeh, S., Abu-El-Ardat, K., Diab-Assaf, M. et al. Epigallocatechin-3-gallate induces apoptosis and cell cycle arrest in HTLV-1-positive and -negative leukemia cells. Med Oncol 25, 30–39 (2008). https://doi.org/10.1007/s12032-007-0036-6

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  • DOI: https://doi.org/10.1007/s12032-007-0036-6

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