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Case–Control Study and Meta-Analysis of the Association Between LIPG rs9958947 SNP and Stroke Risk

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Abstract

The rs9958947 single nucleotide polymorphism (SNP) resides in the promoter region of the lipase G (LIPG) gene. This newly discovered SNP increases the risk of stroke in some Asian populations, including Chinese and Korean populations. Stroke is one of the top 5 leading causes of death in Malaysia, so it is of interest to investigate whether this SNP is associated with stroke risk in the Malaysian population. Therefore, this study investigates this association through a case–control study on a Malaysian population along with a comprehensive meta-analysis. Genotyping of LIPG rs9958947 SNP was performed for 241 Malaysians using real-time polymerase chain reaction, and the odds ratios (OR) with 95% confidence intervals were calculated. The meta-analysis was conducted using the software Comprehensive Meta-Analysis ver. 2.2.064. A p value less than 0.05 was considered statistically significant. We observed that the mean age of Malaysian stroke patients was less than that of stroke patients from Korea and China. The meta-analysis showed that the LIPG rs9958947 SNP was significantly associated with an increased risk of ischemic stroke in Asian populations (dominant (CC vs. CT + TT): OR = 1.45, p < 0.001; allelic (C vs. T): OR = 1.21, p = 0.001; heterozygous (CC vs. CT): OR = 1.47, p < 0.001, and homozygous (CC vs. TT): OR = 1.46, p = 0.047). However, there was no evidence to associate this SNP with stroke risk in the Malaysian population (overall CC vs. CT: OR = 1.04, CC vs. TT: OR = 1.25, CC vs. CT + TT, OR = 1.13; all p > 0.05) and blood lipid levels.

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Data Availability

The datasets analyzed in this study are available from the corresponding author on request.

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Funding

This study is financially supported by the Ministry of Higher Education, Malaysia (TRGS0006-SG-2/2014) and Universiti Malaysia Sabah (GUG0128-1/2017).

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Authors and Affiliations

  1. Biotechnology Programme, Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia

    Kok Yeow Phneh, Eric Tzyy Jiann Chong & Ping-Chin Lee

  2. Department of Rehabilitation Medicine, Queen Elizabeth Hospital, Jalan Penampang, 88200, Kota Kinabalu, Sabah, Malaysia

    Syahiskandar Sybil Shah, Elyana Jalil & Chek Siang Kelvin Cheng

  3. Neurology Unit, Queen Elizabeth Hospital, Jalan Penampang, 88200, Kota Kinabalu, Sabah, Malaysia

    Yuen Kang Chia

  4. Faculty of Psychology and Education, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia

    Dayang Maryama Bte Awang Daud

Authors
  1. Kok Yeow Phneh
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  2. Eric Tzyy Jiann Chong
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  3. Syahiskandar Sybil Shah
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  4. Yuen Kang Chia
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  5. Dayang Maryama Bte Awang Daud
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  6. Elyana Jalil
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  7. Chek Siang Kelvin Cheng
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  8. Ping-Chin Lee
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Contributions

PCL and ETJC conceived and designed the study. KYP, ETJC, SSS, YKC, DMAD, EJ, CSKC, and PCL collected the data. KYP and ETJC conducted the experiments, analyzed the data, and drafted the manuscript. PCL revised the final version of the manuscript. All authors approved the final version of the manuscript.

Corresponding author

Correspondence to Ping-Chin Lee.

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Competing Interests

The authors declare that there is no conflict of interest.

Ethics Approval

This study was approved by the Medical Research and Ethics Committee of the Ministry of Health Malaysia with reference no: NMRR-16–38-28777 (IIR). The procedures used in this study adhere to the tenets of the Declaration of Helsinki.

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Informed consent was obtained from all individual participants included in the study.

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Phneh, K.Y., Chong, E.T.J., Shah, S.S. et al. Case–Control Study and Meta-Analysis of the Association Between LIPG rs9958947 SNP and Stroke Risk. J Mol Neurosci 71, 2085–2094 (2021). https://doi.org/10.1007/s12031-021-01795-w

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  • DOI: https://doi.org/10.1007/s12031-021-01795-w

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