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Knockdown of PFTK1 Inhibits the Migration of Glioma Cells

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Abstract

The prognosis of glioma patients is generally poor, so it is urgent to find out the underlying molecular mechanisms. PFTK1 is a member of cyclin-dependent kinases (Cdks) family and has been reported to contribute to tumor migration and invasion. In this study, we aimed to explore the expression and function in human glioma. Western blot and immunohistochemistry were used to evaluate the expression of PFTK1. PFTK1 expression was higher in glioma tissues compared with normal brain tissues, and its level was associated with the WHO grade in Western blot analysis. The suppression of PFTK1 expression by RNA interference was shown to inhibit the migration of glioma cells. Knockdown of PFTK1 increases E-cadherin expression and decreases vimentin expression. These data show that PFTK1 may participate in the pathogenic process of glioma, suggesting that PFTK1 can become a potential therapeutic strategy for gastric cancer.

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Acknowledgments

This work was supported by the National Basic Research Program of China (973 Program, No. 2012CB822104), the National Natural Science Foundation of China (No. 81202368), and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. Department of Neurosurgery, The Affiliated Hospital of Nantong University, Xisi Road No. 20, Nantong, 226001, People’s Republic of China

    Shaochen Fan & Yilu Gao

  2. Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226001, People’s Republic of China

    Shaochen Fan & Yilu Gao

  3. Department of Neurosurgery, Nantong Second People Affiliated Hospital of Nantong University, 43 Xinglong Road, Nantong, 226001, Jiangsu Province, People’s Republic of China

    Chengjin Zhao, Shirong Dai, Jianbing Ren & Xiubing Zhang

  4. Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province, 226001, People’s Republic of China

    Li Zhang, Na Ban, Xiaojuan He, Wenjuan Chen, Jie Sun & Tao Tao

  5. Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, 215006, People’s Republic of China

    Lixiang Yang & Zhen Bao

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  1. Shaochen Fan
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  2. Chengjin Zhao
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  3. Li Zhang
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  7. Na Ban
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  10. Zhen Bao
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  11. Wenjuan Chen
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  12. Jie Sun
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  13. Yilu Gao
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  14. Tao Tao
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Corresponding authors

Correspondence to Yilu Gao or Tao Tao.

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Shaochen Fan and Chengjin Zhao have contributed equally to this work.

Tao Tao and Yilu Gao have contributed equally to this work.

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Fan, S., Zhao, C., Zhang, L. et al. Knockdown of PFTK1 Inhibits the Migration of Glioma Cells. J Mol Neurosci 57, 257–264 (2015). https://doi.org/10.1007/s12031-015-0600-z

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  • DOI: https://doi.org/10.1007/s12031-015-0600-z

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