Abstract
We sought to develop an immunohistochemical (IHC) tool to support the diagnosis of parathyroid carcinoma (PC) and help differentiate it from atypical parathyroid neoplasms (atypical) and benign adenomas. Distinguishing PC from benign parathyroid neoplasms can be challenging. Many cases of PC are histopathologically borderline for definitive malignancy. Recently, individual IHC biomarkers have been evaluated to aid in discrimination between parathyroid neoplasms. PC, atypical parathyroid neoplasms, and parathyroid adenomas treated at our institution from 1997 to 2014 were studied retrospectively. IHC analysis was performed to evaluate parafibromin, retinoblastoma (RB), protein gene product 9.5 (PGP9.5), Ki67, galectin-3, and E-cadherin expression. Receiver operating characteristic (ROC) analysis and multivariable logistic regression model for combinations of biomarkers were evaluated to classify patients as PC or atypical/adenoma. A diagnostic nomogram using 5 biomarkers was created for PC. Sixty-three patients were evaluated. The percent staining of parafibromin (p < 0.0001), RB (p = 0.04), Ki67 (p = 0.02), PGP9.5 (p = 0.04), and Galectin-3 (p = 0.01) differed significantly in the three diagnostic groups. ROC analysis demonstrated that parafibromin had the best performance in discriminating PC from atypical/adenoma; area under the curve (AUC) was 81% (cutoff, 92.5%; sensitivity rate, 64%; specificity rate, 87%). We created a diagnostic nomogram using a combination of biomarkers; AUC was 84.9% (95% confidence interval, 73.4-96.4%). The optimism-adjusted AUC for this model was 80.5% (mean absolute error, 0.043). A diagnostic nomogram utilizing an immunoexpression, a combination of immunohistochemical biomarkers, can be used to help differentiate PC from other parathyroid neoplasms, thus potentially improving diagnostic classification.
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References
G. G. Fernandez-Ranvier et al., “Defining a molecular phenotype for benign and malignant parathyroid tumors,” Cancer, vol. 115, no. 2, pp. 334-344, Jan 15 2009, https://doi.org/10.1002/cncr.24037.
A. Mohebati, A. Shaha, and J. Shah, “Parathyroid carcinoma: challenges in diagnosis and treatment,” Hematology/oncology clinics of North America, vol. 26, no. 6, pp. 1221-1238, Dec 2012, https://doi.org/10.1016/j.hoc.2012.08.009.
K. Duan and O. Mete, “Parathyroid Carcinoma: Diagnosis and Clinical Implications,” Turk Patoloji Derg, vol. 31 Suppl 1, pp. 80-97, 2015, https://doi.org/10.5146/tjpath.2015.01316.
R. A. DeLellis, C. Larsson, A. Arnold, R. Lloy, J. Bilezikian, and O. Mete, “Tumors of the parathyroid glands,” in In WHO Classification of Tumors of Endocrine Organs, R. R Lloyd, Osamura, G., Kloppel & J Rosai. Ed. Lyon, France: IARC Press, 2017, pp. pp 145–159.
G. G. Fernandez-Ranvier et al., “Parathyroid carcinoma, atypical parathyroid adenoma, or parathyromatosis?,” Cancer, vol. 110, no. 2, pp. 255-264, Jul 15 2007, https://doi.org/10.1002/cncr.22790.
J. Chen, J. Liu, and J. Wang, “[Diagnosis and surgical treatment of parathyroid carcinoma],” Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery, vol. 24, no. 12, pp. 541-544, Jun 2010.
I. Christakis, N. Bussaidy, C. Clarke, L. J. Kwatampora, C. L. Warneke, A. M. Silva, M. D. Williams, E. G. Grubbs, J. E. Lee, N. D. Perrier, “Differentiating Atypical Parathyroid Neoplasm from Parathyroid Cancer (in English),” Annals of surgical oncology, vol. 23, no. 9, pp. 2889-2897, Sep 2016, https://doi.org/10.1245/s10434-016-5248-6.
C. A. Wang and R. D. Gaz, “Natural history of parathyroid carcinoma. Diagnosis, treatment, and results,” American journal of surgery, vol. 149, no. 4, pp. 522-527, Apr 1985.
R. A. Delellis, “Challenging lesions in the differential diagnosis of endocrine tumors: parathyroid carcinoma,” Endocr Pathol, vol. 19, no. 4, pp. 221-225, Winter 2008, https://doi.org/10.1007/s12022-008-9050-2.
K. Duan, K. Gomez Hernandez, and O. Mete, “Clinicopathological correlates of hyperparathyroidism,” J Clin Pathol, Jul 10 2015, https://doi.org/10.1136/jclinpath-2015-203186.
L. A. Erickson and O. Mete, “Immunohistochemistry in Diagnostic Parathyroid Pathology,” Endocr Pathol, vol. 29, no. 2, pp. 113-129, Jun 2018, https://doi.org/10.1007/s12022-018-9527-6.
C. C. Juhlin et al., “Parafibromin immunoreactivity: its use as an additional diagnostic marker for parathyroid tumor classification,” Endocr Relat Cancer, vol. 14, no. 2, pp. 501-512, Jun 2007, https://doi.org/10.1677/ERC-07-0021.
O. Wang et al., “Expression of Ki-67, galectin-3, fragile histidine triad, and parafibromin in malignant and benign parathyroid tumors (in English), Chinese Medical Journal, vol. 125, no. 16, pp. 2895-2901, Aug 20 2012, https://doi.org/10.3760/cma.j.issn.0366-6999.2012.16.018.
V. Guarnieri et al., “CDC73 mutations and parafibromin immunohistochemistry in parathyroid tumors: clinical correlations in a single-centre patient cohort,” Cell Oncol (Dordr), vol. 35, no. 6, pp. 411-422, Dec 2012, https://doi.org/10.1007/s13402-012-0100-x.
P. P. Truran, S. J. Johnson, R. D. Bliss, T. W. Lennard, and S. R. Aspinall, “Parafibromin, galectin-3, PGP9.5, Ki67, and cyclin D1: using an immunohistochemical panel to aid in the diagnosis of parathyroid cancer,” World J Surg, vol. 38, no. 11, pp. 2845-2854, Nov 2014, https://doi.org/10.1007/s00268-014-2700-2.
S. Kruijff, S. B. Sidhu, M. S. Sywak, A. J. Gill, and L. W. Delbridge, “Negative Parafibromin Staining Predicts Malignant Behavior in Atypical Parathyroid Adenomas,” (in English), Annals of Surgical Oncology, vol. 21, no. 2, pp. 426-433, Feb 2014, https://doi.org/10.1245/s10434-013-3288-8.
C. C. Juhlin, I.L. Nilsson, K. Lagerstedt-Robinson, A. Stenman, R. Bränström, E. Tham, A. Höög, “Parafibromin immunostainings of parathyroid tumors in clinical routine: a near-decade experience from a tertiary center,” Mod Pathol, vol. 32, no. 8, pp. 1082-1094, Jul 2019, https://doi.org/10.1038/s41379-019-0252-6.
S. Karaarslan et al., “The Role of Parafibromin, Galectin-3, HBME-1, and Ki-67 in the Differential Diagnosis of Parathyroid Tumors,” Oman Med J, vol. 30, no. 6, pp. 421-427, Nov 2015, https://doi.org/10.5001/omj.2015.84.
K. Hosny Mohammed et al., “Parafibromin, APC, and MIB-1 Are Useful Markers for Distinguishing Parathyroid Carcinomas From Adenomas,’ Appl Immunohistochem Mol Morphol, vol. 25, no. 10, pp. 731-735, Nov/Dec 2017, https://doi.org/10.1097/PAI.0000000000000378.
V. M. Howell et al., “Accuracy of combined protein gene product 9.5 and parafibromin markers for immunohistochemical diagnosis of parathyroid carcinoma,” The Journal of clinical endocrinology and metabolism, vol. 94, no. 2, pp. 434-441, Feb 2009, https://doi.org/10.1210/jc.2008-1740.
N. Kumari, N. Chaudhary, R. Pradhan, A. Agarwal, and N. Krishnani, “Role of Histological Criteria and Immunohistochemical Markers in Predicting Risk of Malignancy in Parathyroid Neoplasms,” Endocr Pathol, vol. 27, no. 2, pp. 87-96, Jun 2016, https://doi.org/10.1007/s12022-016-9426-7.
N. Bergero et al., “Galectin-3 expression in parathyroid carcinoma: immunohistochemical study of 26 cases,” Hum Pathol, vol. 36, no. 8, pp. 908-914, Aug 2005, https://doi.org/10.1016/j.humpath.2005.06.020.
F. Lumachi et al., “PCNA-LII, Ki-67 immunostaining, p53 activity and histopathological variables in predicting the clinical outcome in patients with parathyroid carcinoma,” Anticancer Res, vol. 26, no. 2A, pp. 1305-1308, Mar-Apr 2006.
V. L. Cryns et al., “Loss of the retinoblastoma tumor-suppressor gene in parathyroid carcinoma,” N Engl J Med, vol. 330, no. 11, pp. 757-761, Mar 17 1994, https://doi.org/10.1056/NEJM199403173301105.
B. M. Erovic, L. Harris, M. Jamali, D. P. Goldstein, J. C. Irish, S. L. Asa, O. Mete, “Biomarkers of parathyroid carcinoma,” Endocr Pathol, vol. 23, no. 4, pp. 221-231, Dec 2012, https://doi.org/10.1007/s12022-012-9222-y.
V. Fendrich et al., “Unique expression pattern of the EMT markers Snail, Twist and E-cadherin in benign and malignant parathyroid neoplasia,” Eur J Endocrinol, vol. 160, no. 4, pp. 695-703, Apr 2009, https://doi.org/10.1530/EJE-08-0662.
F. Cetani, C. Marcocci, L. Torregrossa, and E. Pardi, “Atypical parathyroid adenomas: challenging lesions in the differential diagnosis of endocrine tumors,’ Endocr Relat Cancer, May 1 2019, https://doi.org/10.1530/ERC-19-0135.
J. D. Carpten et al., “HRPT2, encoding parafibromin, is mutated in hyperparathyroidism-jaw tumor syndrome,” Nat Genet, vol. 32, no. 4, pp. 676-680, Dec 2002, https://doi.org/10.1038/ng1048.
V. M. Howell et al., “HRPT2 mutations are associated with malignancy in sporadic parathyroid tumours,” J Med Genet, vol. 40, no. 9, pp. 657-663, Sep 2003.
T. M. Shattuck et al., “Somatic and germ-line mutations of the HRPT2 gene in sporadic parathyroid carcinoma,” N Engl J Med, vol. 349, no. 18, pp. 1722-1729, Oct 30 2003, https://doi.org/10.1056/NEJMoa031237.
F. Cetani et al., “Genetic analyses of the HRPT2 gene in primary hyperparathyroidism: germline and somatic mutations in familial and sporadic parathyroid tumors,” The Journal of clinical endocrinology and metabolism, vol. 89, no. 11, pp. 5583-5591, Nov 2004, https://doi.org/10.1210/jc.2004-0294.
A. J. Gill et al., “Loss of nuclear expression of parafibromin distinguishes parathyroid carcinomas and hyperparathyroidism-jaw tumor (HPT-JT) syndrome-related adenomas from sporadic parathyroid adenomas and hyperplasias,” Am J Surg Pathol, vol. 30, no. 9, pp. 1140-9, Sep 2006, https://doi.org/10.1097/01.pas.0000209827.39477.4f.
C. J. Haven et al., “Identification of MEN1 and HRPT2 somatic mutations in paraffin-embedded (sporadic) parathyroid carcinomas,” Clinical endocrinology, vol. 67, no. 3, pp. 370-376, Sep 2007, https://doi.org/10.1111/j.1365-2265.2007.02894.x.
F. Cetani et al., ”Should parafibromin staining replace HRTP2 gene analysis as an additional tool for histologic diagnosis of parathyroid carcinoma? (in English), European journal of endocrinology/European Federation of Endocrine Societies, vol. 156, no. 5, pp. 547-554, 2007.
W. F. Benedict, H. J. Xu, S. X. Hu, and R. Takahashi, “Role of the retinoblastoma gene in the initiation and progression of human cancer,” J Clin Invest, vol. 85, no. 4, pp. 988-993, Apr 1990, https://doi.org/10.1172/JCI114575.
A. Giordano and H. E. Kaiser, “The retinoblastoma gene: Its role in cell cycle and cancer (in English),” In Vivo, vol. 10, no. 2, pp. 223-227, Mar-Apr 1996.
M. Classon and E. Harlow, “The retinoblastoma tumour suppressor in development and cancer (in English),” Nature Reviews Cancer, vol. 2, no. 12, pp. 910-917, Dec 2002, https://doi.org/10.1038/nrc950.
W. R. Sellers and W. G. Kaelin, “Role of the retinoblastoma protein in the pathogenesis of human cancer (in English),” Journal of Clinical Oncology, vol. 15, no. 11, pp. 3301-3312, Nov 1997.
P. Subramaniam, S. Wilkinson, and J. J. Shepherd, “Inactivation of retinoblastoma gene in malignant parathyroid growths: a candidate genetic trigger?,” Aust N Z J Surg, vol. 65, no. 10, pp. 714-716, Oct 1995.
W. Birchmeier and J. Behrens, “Cadherin Expression in Carcinomas - Role in the Formation of Cell-Junctions and the Prevention of Invasiveness (in English), Bba-Rev Cancer, vol. 1198, no. 1, pp. 11-26, May 27 1994, https://doi.org/10.1016/0304-419x(94)90003-5.
R. Schneider, S. Bartsch-Herzog, A. Ramaswamy, D. K. Bartsch, and E. Karakas, “Immunohistochemical Expression of E-Cadherin in Atypical Parathyroid Adenoma,” World journal of surgery, Jul 8 2015, https://doi.org/10.1007/s00268-015-3149-7.
T. Scholzen and J. Gerdes, “The Ki-67 protein: From the known and the unknown (in English), J Cell Physiol, vol. 182, no. 3, pp. 311-322, Mar 2000, https://doi.org/10.1002/(Sici)1097-4652(200003)182:3<311::Aid-Jcp1>3.0.Co;2-9.
G. C. Abbona, M. Papotti, G. Gasparri, and G. Bussolati, “Proliferative Activity in Parathyroid Tumors as Detected by Ki-67 Immunostaining (in English), Human Pathology, vol. 26, no. 2, pp. 135-138, Feb 1995, https://doi.org/10.1016/0046-8177(95)90028-4.
A. Stojadinovic et al., “Parathyroid neoplasms: clinical, histopathological, and tissue microarray-based molecular analysis,” Hum Pathol, vol. 34, no. 1, pp. 54-64, Jan 2003, https://doi.org/10.1053/hupa.2003.55.
K. Arvai et al., “Molecular profiling of parathyroid hyperplasia, adenoma and carcinoma,” Pathology oncology research : POR, vol. 18, no. 3, pp. 607-614, Jul 2012, https://doi.org/10.1007/s12253-011-9483-7.
Y. Tokumaru et al., “The role of PGP9.5 as a tumor suppressor gene in human cancer (in English), Int J Cancer, vol. 123, no. 4, pp. 753-759, Aug 15 2008, https://doi.org/10.1002/ijc.23354.
M. Hochstrasser, “Ubiquitin-dependent protein degradation (in English), Annu Rev Genet, vol. 30, pp. 405-439, 1996, https://doi.org/10.1146/annurev.genet.30.1.405.
A. Agarwal, R. Pradhan, N. Kumari, N. Krishnani, P. Shukla, S. K. Gupta, G. Chand, A. Mishra, G. Agarwal, A. K. Verma, S. K. Mishra, “Molecular Characteristics of Large Parathyroid Adenomas,” World journal of surgery, vol. 40, no. 3, pp. 607-614, Mar 2016, https://doi.org/10.1007/s00268-015-3380-2.
F. T. Liu and G. A. Rabinovich, “Galectins as modulators of tumour progression (in English),” Nature Reviews Cancer, vol. 5, no. 1, pp. 29-41, Jan 2005, https://doi.org/10.1038/nrc1527.
E. Saggiorato et al., “Galectin-3 and Ki-67 expression in multiglandular parathyroid lesions,” Am J Clin Pathol, vol. 126, no. 1, pp. 59-66, Jul 2006, https://doi.org/10.1309/9NXP-7FRF-87MU-2PCK.
G. Ippolito, F. Sebag, F. Palazzo, E. Trichet, C. De Micco, and J. F. Henry, “Parathyroid cancer and atypical adenoma: operational diagnosis and surgical treatment (in French), Diabetes Metab, vol. 33, pp. S63-S63, Mar 2007.
K. L. Mccoy et al., “The clinical importance of parathyroid atypia: Is long-term surveillance necessary? (in English),” Surgery, vol. 158, no. 4, pp. 929-935, Oct 2015, https://doi.org/10.1016/j.surg.2015.06.022.
F. Cetani, E. Pardi, and C. Marcocci, “Update on parathyroid carcinoma,” Journal of endocrinological investigation, Mar 21 2016, https://doi.org/10.1007/s40618-016-0447-3.
A. M. Silva-Figueroa et al., “Prognostic Scoring System to Risk Stratify Parathyroid Carcinoma,” J Am Coll Surg, Mar 15 2017, https://doi.org/10.1016/j.jamcollsurg.2017.01.060.
A. J. Gill et al., “Parafibromin-deficient (HPT-JT Type, CDC73 Mutated) Parathyroid Tumors Demonstrate Distinctive Morphologic Features,” Am J Surg Pathol, vol. 43, no. 1, pp. 35-46, Jan 2019, https://doi.org/10.1097/PAS.0000000000001017.
Y. Hu, Q. Liao, S. Cao, X. Gao, and Y. Zhao, “Diagnostic performance of parafibromin immunohistochemical staining for sporadic parathyroid carcinoma: a meta-analysis,” Endocrine, Jun 1 2016, https://doi.org/10.1007/s12020-016-0997-3.
M. Nakamura et al., “Involvement of galectin-3 expression in colorectal cancer progression and metastasis,” Int J Oncol, vol. 15, no. 1, pp. 143-148, Jul 1999.
K. Kawachi et al., “Galectin-3 expression in various thyroid neoplasms and its possible role in metastasis formation,” Hum Pathol, vol. 31, no. 4, pp. 428-433, Apr 2000, https://doi.org/10.1053/hp.2000.6534.
F. Cetani et al., “A reappraisal of the Rb1 gene abnormalities in the diagnosis of parathyroid cancer,” Clin Endocrinol (Oxf), vol. 60, no. 1, pp. 99-106, Jan 2004.
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This research was supported in part by Cancer Center Support Grant (NCI Grant P30 CA016672).
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Silva-Figueroa, A.M., Bassett, R., Christakis, I. et al. Using a Novel Diagnostic Nomogram to Differentiate Malignant from Benign Parathyroid Neoplasms. Endocr Pathol 30, 285–296 (2019). https://doi.org/10.1007/s12022-019-09592-3
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DOI: https://doi.org/10.1007/s12022-019-09592-3