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USP10 Expression in Normal Adrenal Gland and Various Adrenal Tumors

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Abstract

Ubiquitin-specific protease 10 (USP10), a novel deubiquitinating enzyme, is associated with androgen receptor transcriptional activity and pathological processes of tumor. However, information between USP10 and the adrenal gland is limited. In particular, the role of USP10 in adrenal tumors has not been elucidated yet. This study aims to investigate the expression of USP10 in the human normal adrenal gland and various adrenal tumors. Tissue samples were obtained from 30 adrenocortical adenomas, nine adrenocortical adenocarcinomas, and 20 pheochromocytomas following laparoscopic surgery. Twenty normal adrenal glands were obtained from kidney surgical resection conducted due to renal cell carcinomas. USP10 expression was investigated on protein levels using immunohistochemistry and on mRNA levels using bioinformatics analysis in the Gene Expression Omnibus (GEO) Datasets. In the 20 cases of normal adrenal glands analyzed, USP10 protein was constantly expressed in situ in the cortex of the adrenal glands, but in the medulla of the gland, only the sustentacular cells were detected positive. In adrenal tumors, detectable levels of USP10 protein were found in 100 % (30/30) adrenocortical adenomas, 88.89 % (8/9) adrenocortical carcinomas, and 10 % (2/20) pheochromocytomas. Bioinformatics analysis did not show a significant difference in USP10 messenger RNA (mRNA) expression between adrenal tumors and normal adrenal gland tissues. A positive USP10 immunoreaction can be useful in distinguishing adrenal cortical tumors from pheochromocytoma.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant Nos. 81571147 and 81301019) and Independent Scientific Research Subject for Young Teachers of Wu Han University (Grant No. 2042015kf0076).

Authors’ Contribution

Zeng Z: Project development and manuscript writing

Zhou ZY, Zhan N, Yuan JP, and Ye BX: Data collection or management

Gu LJ, Wang J, and Jian ZH: Data analysis

Xiong XX: Protocol development and manuscript editing

Author information

Authors and Affiliations

  1. Department of Pathology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, People’s Republic of China

    Zhi Zeng, Ziying Zhou, Na Zhan & Jingping Yuan

  2. Department of Hematology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, People’s Republic of China

    Baixin Ye

  3. Central Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, People’s Republic of China

    Lijuan Gu & Xiaoxing Xiong

  4. Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, People’s Republic of China

    Jun Wang, Zhihong Jian & Xiaoxing Xiong

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  1. Zhi Zeng
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  2. Ziying Zhou
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  3. Na Zhan
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  4. Jingping Yuan
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  7. Jun Wang
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  9. Xiaoxing Xiong
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Corresponding author

Correspondence to Xiaoxing Xiong.

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The study was approved by the ethics committee of Renmin Hospital of the Wuhan University and was performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments.

Conflict of Interest

The authors declare that they have no competing interests.

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Zeng, Z., Zhou, Z., Zhan, N. et al. USP10 Expression in Normal Adrenal Gland and Various Adrenal Tumors. Endocr Pathol 26, 302–308 (2015). https://doi.org/10.1007/s12022-015-9406-3

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  • DOI: https://doi.org/10.1007/s12022-015-9406-3

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