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Estrogenic regulation of gene and protein expression within the amygdala of female mice

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Abstract

Estrogens exert important actions on fear and anxiety in both humans and non-humans. Currently, the mechanisms underlying estrogenic modulation of fear are not known. However, evidence suggests that estrogens may exert their influence on fear and anxiety within the amygdala. The purpose of the present study was to examine the genomic effects of estrogens within the amygdala of female mice using high-density oligonucleotide microarrays. We examined the effects of estrogens on gene expression at 2 and 24 h after an acute subcutaneous injection. Data from the microarrays revealed that 2 h following an acute injection of estradiol, 44 genes were significantly up- or downregulated, and at 24 h, 13 transcripts were significantly up- or downregulated. One interesting estrogen-regulated gene, (CaMKIIα), was downregulated ninefold 2 h following an acute estradiol injection but was not altered 24 h after injection. We further examined estrogen regulation of CaMKIIα, as well as CaMKIIβ and CaMKIV within the amygdala using quantitative PCR and western blot analysis. The data indicate that estrogen decreases CaMKIIα and CaMKIV but not CaMKIIβ gene expression within the amygdala. However, CaMKII protein levels were not different, and CaMKIV protein levels increased 2 h post-EB treatment. These results indicate that estrogen regulates CaMK gene expression and protein levels within the amygdala.

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References

  1. N. Breslau, G.C. Davis, P. Andreski, E. Peterson, Arch. Gen. Psychiatr. 48, 216–222 (1991)

    CAS  PubMed  Google Scholar 

  2. D.A. Regier, W.E. Narrow, D.S. Rae, R.W. Manderscheid, B.Z. Locke, F.K. Goodwin, Arch. Gen. Psychiatr. 50, 85–94 (1993)

    CAS  PubMed  Google Scholar 

  3. J.C. Arpels, J. Reprod. Med. 41, 633–639 (1996)

    CAS  PubMed  Google Scholar 

  4. S. Campbell, M. Whitehead, Clin. Obstet. Gynaecol. 4, 31–47 (1977)

    CAS  PubMed  Google Scholar 

  5. B.B. Sherwin, Proc. Soc. Exp. Biol. Med. 217, 17–22 (1998)

    CAS  PubMed  Google Scholar 

  6. D.A. Sichel, L.S. Cohen, L.M. Robertson, A. Ruttenberg, J.F. Rosenbaum, Biol. Psychiatr. 38, 814–818 (1995)

    Article  CAS  Google Scholar 

  7. H. Shibata, T.E. Spencer, S.A. Onate, G. Jenster, S.Y. Tsai, M.J. Tsai, P.J. Scrimo, B.W. O’Malley, Recent Prog. Horm. Res. 52, 141–164 (1997)

    CAS  PubMed  Google Scholar 

  8. C.L. Smith, S.A. Onate, M.J. Tsai, B.W. O’Malley, Proc. Natl Acad. Sci. USA 93, 8884–8888 (1996)

    Article  CAS  PubMed  Google Scholar 

  9. J.L. Turgeon, D.P. McDonnell K.A. Martin, P.M. Wise, Science 304, 1269–1273 (2004)

    Article  CAS  PubMed  Google Scholar 

  10. J.J. Watters, D.M. Dorsa, J. Neurosci. 18, 6672–6680 (1998)

    CAS  PubMed  Google Scholar 

  11. R. Bi, G. Broutman, M.R. Foy, R.F. Thompson, M. Baudry, Proc. Natl Acad. Sci. USA 97, 3602–3607 (2000)

    Article  CAS  PubMed  Google Scholar 

  12. R.D. Brinton, Learn. Mem. 8, 121–133 (2001)

    Article  CAS  PubMed  Google Scholar 

  13. M.J. Kelly, E.R. Levin, Trends Endocrinol. Metab. 12, 152–156 (2001)

    Article  CAS  PubMed  Google Scholar 

  14. M.D. Womble, J.A. Andrew, J.J. Crook, Neurosci. Lett. 331, 83–86 (2002)

    Article  CAS  PubMed  Google Scholar 

  15. N. Vasudevan, L.M. Kow, D.W. Pfaff, Proc. Natl Acad. Sci. USA 98, 12267–12271 (2001)

    Article  CAS  PubMed  Google Scholar 

  16. M. Davis, The Amygdala, 2nd edn. (2000), pp. 213–287

  17. J.E. LeDoux, Annu. Rev. Neurosci. 23, 155–184 (2000)

    Article  CAS  PubMed  Google Scholar 

  18. N. Laflamme, R.E. Nappi, G. Drolet, C. Labrie, S. Rivest, J. Neurobiol. 36, 357–378 (1998)

    Article  CAS  PubMed  Google Scholar 

  19. I. Merchenthaler, M.V. Lane, S. Numan, T.L. Dellovade, J. Comp. Neurol. 473, 270–291 (2004)

    Article  CAS  PubMed  Google Scholar 

  20. M. Osterlund, G.G. Kuiper, J.A. Gustafsson, Y.L. Hurd, Brain Res. Mol. Brain Res. 54, 175–180 (1998)

    Article  CAS  PubMed  Google Scholar 

  21. M.K. Osterlund, E. Keller, Y.L. Hurd, Neuroscience 95, 333–342 (2000)

    Article  CAS  PubMed  Google Scholar 

  22. P.J. Shughrue, P.J. Scrimo, I. Merchenthaler, Endocrinology 139, 5267–5270 (1998)

    Article  CAS  PubMed  Google Scholar 

  23. A.M. Jasnow, J. Schulkin, D.W. Pfaff, Horm. Behav. 49, 197–205 (2006)

    Article  CAS  PubMed  Google Scholar 

  24. M.A. Morgan, D.W. Pfaff, Horm. Behav. 40, 472–482 (2001)

    Article  CAS  PubMed  Google Scholar 

  25. S.A. Bustin, J. Mol. Endocrinol. 25, 169–193 (2000)

    Article  CAS  PubMed  Google Scholar 

  26. S.A. Bustin, J. Mol. Endocrinol. 29, 23–39 (2002)

    Article  CAS  PubMed  Google Scholar 

  27. S.S. Hook, A.R. Means, Annu. Rev. Pharmacol. Toxicol. 41, 471–505 (2001)

    Article  CAS  PubMed  Google Scholar 

  28. L.C. Griffith, J. Neurosci. 24, 8391–8393 (2004)

    Article  CAS  PubMed  Google Scholar 

  29. C. Chen, D.G. Rainnie, R.W. Greene, S Tonegawa, Science 266, 291–294 (1994)

    Article  CAS  PubMed  Google Scholar 

  30. L. Plumari, Viglietti-Panzica, C., F. Allieri, S. Honda, N. Harada, P. Absil, J. Balthazart, G.C. Panzica, J. Neuroendocrinol. 14, 971–978 (2002)

    Article  CAS  PubMed  Google Scholar 

  31. E.M. Scordalakes, E.F. Rissman, Genes Brain Behav. 3, 20–26 (2004)

    Article  CAS  PubMed  Google Scholar 

  32. M. Cammarota, L.R. Bevilaqua, H. Viola, D.S. Kerr, B. Reichmann, V. Teixeira, M. Bulla, I. Izquierdo, J.H. Medina, Cell. Mol. Neurobiol. 22, 259–267 (2002)

    Article  CAS  PubMed  Google Scholar 

  33. J. Lisman, H. Schulman, H. Cline, Nat. Rev. Neurosci. 3, 175–190 (2002)

    Article  CAS  PubMed  Google Scholar 

  34. M. Mayford, M.E. Bach, Y.Y. Huang, L. Wang, R.D. Hawkins, E.R. Kandel, Science 274, 1678–1683 (1996)

    Article  CAS  PubMed  Google Scholar 

  35. S. Miller, M. Yasuda, J.K. Coats, Y. Jones, M.E. Martone, M. Mayford, Neuron 36, 507–519 (2002)

    Article  CAS  PubMed  Google Scholar 

  36. M.A. Morgan, D.W. Pfaff, Behav. Brain Res. 132, 85–93 (2002)

    Article  CAS  PubMed  Google Scholar 

  37. M.A. Morgan, J. Schulkin, D.W. Pfaff, Neurosci. Biobehav. Rev. 28, 55–63 (2004)

    Article  CAS  PubMed  Google Scholar 

  38. J. Zhou, R.S. Cohen, S.C. Pandey, NeuroReport 12, 2987–2990 (2001)

    Article  CAS  PubMed  Google Scholar 

  39. M. Blurton-Jones, M.H. Tuszynski, J. Comp. Neurol. 452, 276–287 (2002)

    Article  PubMed  Google Scholar 

  40. A.J. McDonald, R.L. Betette, Neuroscience 102, 413–425 (2001)

    Article  CAS  PubMed  Google Scholar 

  41. Y. Smith, J.F. Pare, D. Pare, J. Comp. Neurol. 391, 164–179 (1998)

    Article  CAS  PubMed  Google Scholar 

  42. H. Sorvari, H. Soininen, L. Paljarvi, K. Karkola, A. Pitkanen, J. Comp. Neurol. 360, 185–212 (1995)

    Article  CAS  PubMed  Google Scholar 

  43. A.J. McDonald, F. Mascagni, Neuroscience 105, 681–693 (2001)

    Article  CAS  PubMed  Google Scholar 

  44. A.J. McDonald, J.F. Muller, F. Mascagni, J. Comp. Neurol. 446, 199–218 (2002)

    Article  CAS  PubMed  Google Scholar 

  45. C.N. Rudick, C.S. Woolley, J. Neurosci. 21, 6532–6543 (2001)

    CAS  PubMed  Google Scholar 

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Acknowledgments

The authors thank Bob Cuevos and Darrel Merrick for their expert animal care, Par Parekh for his technical assistance, and Dr Oliver Fricke for thoughtfull discussions during the preparation of the manuscript. This work was supported by MH 070086 to AMJ.

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Authors and Affiliations

  1. Laboratory of Neurobiology and Behavior, The Rockefeller University, New York, NY, 10021, USA

    Aaron M. Jasnow, Jessica A. Mong & Donald W. Pfaff

  2. Harold and Margaret Milliken Hatch Laboratory of Neuroendocrinology, The Rockefeller University, New York, NY, 10021, USA

    Russell D. Romeo

  3. Emory University, Yerkes Neuroscience Building Rm 5224, 954 Gatewood RD NE, Atlanta, GA, 30329, USA

    Aaron M. Jasnow

Authors
  1. Aaron M. Jasnow
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  2. Jessica A. Mong
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  3. Russell D. Romeo
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  4. Donald W. Pfaff
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Correspondence to Aaron M. Jasnow.

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Jasnow, A.M., Mong, J.A., Romeo, R.D. et al. Estrogenic regulation of gene and protein expression within the amygdala of female mice. Endocr 32, 271–279 (2007). https://doi.org/10.1007/s12020-008-9043-4

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  • DOI: https://doi.org/10.1007/s12020-008-9043-4

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