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Dexamethasone-induced suppression of steroidogenic acute regulatory protein gene expression in mouse Y-1 adrenocortical cells is associated with reduced histone H3 acetylation

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Abstract

In this study, we investigated the effect of dexamethasone on the expression of steroidogenic acute regulatory protein (StAR) and the acetylation of histone H3 in mouse Y-1 adrenocortical tumor cells. Treatment of Y-1 cells with increasing concentrations (0.001–50 μg/ml) of dexamethasone for 24 h suppressed 8-Br-cAMP (0.5 mM)-stimulated StAR mRNA and protein levels and progesterone production in a dose-dependent manner. Treatment of Y-1 cells with 8-Br-cAMP (0.5 mM) for 1–24 h resulted in a marked increase in StAR mRNA levels. This increase was associated with an increase in progesterone production. StAR mRNA was down-regulated by dexamethasone at times greater than 3 h. To evaluate dexamethasone effect on the endogenous StAR gene, chromatin immunoprecipitation assays were performed in combination with polymerase chain reaction. 8-Br-cAMP increased histone H3 acetylation within the proximal region of the StAR gene promoter and coincubation with dexamethasone blocked this effect. Dexamethasone had no effect on glucocorticoid receptor mRNA expression. These results demonstrate that dexamethasone repression of 8-Br-cAMP-stimulated StAR gene expression in Y-1 cells is accompanied by reductions in histone H3 acetylation associated with the StAR gene promoter.

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References

  1. C.D. Hill, B. Singer, J. Endocrinol. 42, 301–309 (1968)

    Article  CAS  PubMed  Google Scholar 

  2. R.V. Carsia, G.J. MacDonald, S. Malamed, Steroids 41, 741–746 (1983)

    Article  CAS  PubMed  Google Scholar 

  3. F.G. Peron, F. Monclon, R.I. Dorfman, Endocrinology 67, 379–388 (1960)

    Article  CAS  PubMed  Google Scholar 

  4. M.A. Stockman, J. Physiol. 173, 149–161 (1964)

    Google Scholar 

  5. L.B. Morrow, G.N. Burrow, P.F. Mulrow, Endocrinology 80, 883–888 (1967)

    Article  CAS  PubMed  Google Scholar 

  6. R.V. Carsia, S. Malamed, Biochim. Biophys. Acta 763, 83–89 (1983)

    Article  CAS  PubMed  Google Scholar 

  7. A.I. Kahri, R. Voutilainen, A.M. Salmenper, Acta Endocrinol. 91, 329–337 (1979)

    CAS  PubMed  Google Scholar 

  8. P.J. Hornsby, Endocrinology 111, 1092–1101 (1982)

    Article  CAS  PubMed  Google Scholar 

  9. E. Saito, I.M. Muka, T. Muraki, Y. Ichikawa, M. Homma, Endocrinology 104, 487–492 (1979)

    Article  CAS  PubMed  Google Scholar 

  10. R.V. Carsia, S. Malamed, Endocrinology 105, 911–914 (1979)

    Article  CAS  PubMed  Google Scholar 

  11. R.V. Carsia, C.G. Scanes, S. Malamed, Endocrinology 115, 2464–2472 (1984)

    Article  CAS  PubMed  Google Scholar 

  12. F. Musajo, G. Neri, C. Tortorella, G. Mazzocchi, G.G. Nussdorfer, Life Sci. 59, 1401–1406 (1996)

    Article  CAS  PubMed  Google Scholar 

  13. D.S. Loose, Y.S. Do, T.L. Chen, D. Feldman, Endocrinology 107, 137–146 (1980)

    Article  CAS  PubMed  Google Scholar 

  14. W.H. Trzeciak, J.G. LeHoux, M.W. Waterman, E.R. Simpson, Mol. Endocrinol. 7, 206–213 (1993)

    Article  CAS  PubMed  Google Scholar 

  15. C.D. Jefcoate, M.J. Bartolomeis, C.A. Williams, B.C. McNamara, J. Steroid Biochem. 27, 721–729 (1987)

    Article  CAS  PubMed  Google Scholar 

  16. B.J. Clark, S.J. Well, S.R. King, D.M. Stocco, J. Biol. Chem. 269, 28314–28322 (1994)

    CAS  PubMed  Google Scholar 

  17. D. Lin, T. Sugawara, J.F. Strauss III, B.J. Clark, D.M. Stocco, P. Saenger, A. Rogol, W.L. Miller, Science 267, 1828–1831 (1995)

    Article  CAS  PubMed  Google Scholar 

  18. T. Hasegawa, L. Zhao, K.M. Caron, G. Majdic, T. Suzuki, S. Shizawa, H. Sasano, K.L. Parker, Mol. Endocrinol. 14, 1462–1471 (2000)

    Article  CAS  PubMed  Google Scholar 

  19. D.M. Stocco, B.J. Clark, Endocr. Rev. 17, 221–244 (1996)

    CAS  PubMed  Google Scholar 

  20. D.M. Stocco, T.C. Sodeman, J. Biol. Chem. 266, 19731–19738 (1991)

    CAS  PubMed  Google Scholar 

  21. B.J. Clark, S.C. Soo, K.M. Caron, Y. Ikeda, K.L. Parker, D.M. Stocco, Mol. Endocrinol. 9, 1346–1355 (1995)

    Article  CAS  PubMed  Google Scholar 

  22. M. Grunstein, Nature 389, 349–352 (1997)

    Article  CAS  PubMed  Google Scholar 

  23. L.K. Christenson, R.L. Stouffer, J.F. Strauss III, J. Biol. Chem. 276, 27392–27399 (2001)

    Article  CAS  PubMed  Google Scholar 

  24. H. Hiroi, L.K. Christenson, L. Chang, M.D. Sammel, S.L. Berger, J.F. Strauss III, Mol. Endocrinol. 18, 791–806 (2004)

    Article  CAS  PubMed  Google Scholar 

  25. M.T. Pham-Huu-Trung, N. de Smitter, A. Bogyo, F. Girard, J. Steroid Biochem. 21, 93–99 (1984)

    Article  CAS  PubMed  Google Scholar 

  26. M. Reincke, B. Allolio, G. Wurth, J. Clin. Endocrinol. Metab. 77, 151–156 (1993)

    Article  CAS  PubMed  Google Scholar 

  27. A. Soni, G.M. Pepper, P.M. Wyrwinski, N.E. Ramirez, R. Simon, T. Pina, H. Gruenspan, C.E. Vaca, Am. J. Med. 98, 266–271 (1995)

    Article  CAS  PubMed  Google Scholar 

  28. S.W. Lamberts, H.A. Bruining, F.H. de Jong, N. Engl. J. Med. 337, 1285–1292 (1997)

    Article  CAS  PubMed  Google Scholar 

  29. M.L. Richards, R.H. Caplan, G.G. Wickus, Surgery 125, 431–440 (1999)

    CAS  PubMed  Google Scholar 

  30. U. Westphal, Arch. Biochem. Biophys. 118, 556–567 (1967)

    Article  CAS  PubMed  Google Scholar 

  31. E.B. Thompson, M.E. Lippman, Metabolism 23, 159–202 (1994)

    Article  Google Scholar 

  32. K.L. Burstein, C.M. Jewell, J.A. Cidlowski, J. Biol. Chem. 265, 7284–7291 (1990)

    Google Scholar 

  33. J.E. Kalinyak, R.I. Dorin, A.R. Hoffman, A.J. Perlman, J. Biol. Chem. 262, 10441–10444 (1987)

    CAS  PubMed  Google Scholar 

  34. S. Rosewicz, A.R. McDonald, B.A. Maddux, I.D. Goldfine, R.L. Miesfed, C.D. Logsdon, J. Biol. Chem. 263, 2581–2584 (1988)

    CAS  PubMed  Google Scholar 

  35. Y. Dong, L. Poellinger, J.A. Gustafsson, S. Okret, Mol. Endocrinol. 2, 1256–1264 (1988)

    Article  CAS  PubMed  Google Scholar 

  36. V. Supornsilchai, O. Soder, K. Svechnikov, Reprod. Toxicol. 22, 631–635 (2006)

    Article  CAS  PubMed  Google Scholar 

  37. C. Mauduit, I. Goddard, V. Basset, E. Tabone, C. Rey, F. Gasnier, F. Dacheux, M. Benahmed, Endocrinology 142, 2509–2520 (2001)

    Article  CAS  PubMed  Google Scholar 

  38. H.B. Bosmann, K.H. Hales, X. Li, Z. Lin, D.M. Stocco, D.R. Hales, Endocrinology 137, 4522–4525 (1996)

    Article  CAS  PubMed  Google Scholar 

  39. T.W. Sandhoff, M.P. McLean, Endocrine 8, 153–160 (1998)

    PubMed  Google Scholar 

  40. C. Le Roy, J.Y. Li, D.M. Stocco, D. Langlois, J.M. Saez, Endocrinology 141, 1599–1607 (2000)

    Article  CAS  PubMed  Google Scholar 

  41. Z. Liu, D.M. Stocco, Endocrinology 138, 2722–2728 (1997)

    Article  CAS  PubMed  Google Scholar 

  42. F. Arakane, S.R. King, Y. Du, C.B. Kallen, L.P. Walsh, H. Watari, D.M. Stocco, J.F. Strauss III, J. Biol. Chem. 272, 32656–32662 (1997)

    Article  CAS  PubMed  Google Scholar 

  43. K.L. Parker, B.P. Schimmer, Endocr. Rev. 18, 261–377 (1997)

    Article  Google Scholar 

  44. M. Ito, R. Yu, J.L. Jameson, Mol. Cell. Biol. 17, 1476–1483 (1997)

    CAS  PubMed  Google Scholar 

  45. E. Lalli, M.H. Melner, D.M. Stocco, P. Sassone-Corsi, Endocrinology 139, 4237–4243 (1998)

    Article  CAS  PubMed  Google Scholar 

  46. B.M. Gummow, J.O. Scheys, V.R. Cancell, G.D. Hammer, Mol. Endocrinol. 20, 2711–2723 (2006)

    Article  CAS  PubMed  Google Scholar 

  47. C.C. Yu, P.S. Li, Endocrine 30, 313–323 (2006)

    Article  CAS  PubMed  Google Scholar 

  48. T. Jenuwein, C.D. Allis, Science 293, 1074–1080 (2001)

    Article  CAS  PubMed  Google Scholar 

  49. M.H. Kuo, C.D. Allis, Methods 19, 425–433 (1999)

    Article  CAS  PubMed  Google Scholar 

  50. R. Rusovici, Y.Y. Hui, H.A. LaVoie, Biol. Reprod. 72, 862–871 (2005)

    Article  CAS  PubMed  Google Scholar 

  51. S.Y. Roth, C.D. Allis, Cell 87, 5–8 (1996)

    Article  CAS  PubMed  Google Scholar 

  52. F.D. Urnov, A.P. Worffe, Oncogene 20, 2991–3006 (2001)

    Article  CAS  PubMed  Google Scholar 

  53. G.J. Narlikar, H.Y. Fan, R.E. Kingston, Cell 108, 475–487 (2002)

    Article  CAS  PubMed  Google Scholar 

  54. K. Struhl, Genes Dev. 12, 599–606 (1998)

    Article  CAS  PubMed  Google Scholar 

  55. K. Ito, P.J. Barnes, I.M. Adcock, Mol. Cell. Biol. 20, 6891–6903 (2000)

    Article  CAS  PubMed  Google Scholar 

  56. L.M. Salvador, Y. Park, J. Cottom, E.T. Maizels, J.C. Jones, R.V. Schillace, D.W. Carr, P. Cheung, C.D. Allis, J.L. Jameson, M. Hunzicker-Dunn, J. Biol. Chem. 276, 40146–40155 (2001)

    Article  CAS  PubMed  Google Scholar 

  57. M.C. Hu, I.C. Gup, J.H. Lin, B.C. Chung, Biochem. J. 274, 813–817 (1991)

    CAS  PubMed  Google Scholar 

  58. H.J. Huang, P.S. Li, Biol. Reprod. 64, 163–170 (2001)

    Article  CAS  PubMed  Google Scholar 

  59. O.H. Lowry, N.J. Rosenbrough, A.I. Farr, R.J. Randall, J. Biol. Chem. 193, 265–275 (1951)

    CAS  PubMed  Google Scholar 

  60. H.S. Huang, M.C. Wu, P.S. Li, J. Cell. Biochem. 79, 58–70 (2000)

    Article  CAS  PubMed  Google Scholar 

  61. S.J. Tsai, M.C. Wiltbank, Biotechniques 21, 862–866 (1996)

    CAS  PubMed  Google Scholar 

  62. X. Li, J. Wong, S.Y. Tsai, M.J. Tsai, B.W. O’Malley, Mol. Cell. Biol. 23, 3763–3773 (2003)

    Article  CAS  PubMed  Google Scholar 

  63. P.S. Li, W.C. Wagner, Biol. Reprod. 29, 11–24 (1983)

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We thank Dr. Hsiao-Sheng Liu for plasmid, Dr. Douglas M. Stocco for antibody and plasmid and Ms. Li-Ming Huang and Mr. Jyh-Chen Lin for technical assistance. This study was supported by a Grant (NSC91-2320-B006-065) from the National Science Council, Republic of China.

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  1. Department of Physiology, College of Medicine, National Cheng Kung University, 1 University Road, Tainan, 70101, Taiwan, ROC

    Wei-Ping Fon & Pi-Hsueh S. Li

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Correspondence to Pi-Hsueh S. Li.

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Fon, WP., Li, PH.S. Dexamethasone-induced suppression of steroidogenic acute regulatory protein gene expression in mouse Y-1 adrenocortical cells is associated with reduced histone H3 acetylation. Endocr 32, 155–165 (2007). https://doi.org/10.1007/s12020-007-9030-1

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