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Glutamine-mediated Modulation of XO/uric acid/NF-kB Signaling Pathway Ameliorates Intestinal I/R-induced Bacterial Translocation and Cardiorenal Inflammatory Injury

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Abstract

This study explored the effect of intestinal ischaemia/reperfusion (I/R) on cardiorenal tissues. The involvement of xanthine oxidase/uric acid/NF-kB signaling in intestinal I/R was also investigated. In addition, the possible protective effect of glutamine was also evaluated. Twenty-four male Wistar rats were acclimatized and then randomly assigned to four groups (n = 6); sham-operated, glutamine-treated rats (GLUT), I/R, and I/R + GLUT. The sham-operated rats were sham-operated and received 0.5 mL of distilled water, GLUT rats were sham-operated and had 1 g/kg b.w. of glutamine, I/R animals had an intestinal I/R procedure and received 0.5 mL of distilled water, and the I/R + GLUT rats had an intestinal I/R procedure and also received 1 g/kg b.w. of glutamine. Treatments were daily and per os. Glutamine attenuated intestinal I/R-induced rise in intestinal and cardiorenal activities of creatinine kinase and lactate dehydrogenase and lactate level. More so, glutamine alleviated I/R-induced rise in malondialdehyde, xanthine oxidase, uric acid, myeloperoxidase, NF-kB, TNF-α, IL-1β, caspase 3 activity, and DNA fragmentation. Furthermore, glutamine suppressed I/R-induced decline in GSH levels and SOD and catalase activities. Moreover, glutamine improved intestinal, cardiac, and renal histology in animals subjected to intestinal I/R.

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Acknowledgements

Authors are thankful to the staff members of The Brainwill Laboratory, Osogbo, Osun State, Nigeria and The Reprodutcive Biology and Toxicology Research Laboratory, Oasis of Grace Hospital, Osun State, Nigeria, for their technical support.

Author contributions

Conceptualization and design: MAH and REA. Data curation: MAH, TMA, and REA. Funding acquisition: MAH, OOA, OIO, TMA, FDF, OCA, TFO, OSA, IAO, and REA. Investigation: MAH, OOA, OIO, TMA, FDF, OCA, TFO, OSA, IAO, and REA. Methodology: REA. Project administration: MAH, OOA, OIO, TMA, FDF, OCA, TFO, OSA, IAO, and REA. Supervision: MAH and REA. Validation: MAH, TMA, and REA. Writing-original draft: TMA and REA. Writing-review and editing and final approval: MAH, OOA, OIO, TMA, FDF, OCA, TFO, OSA, IAO, and REA.

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Authors and Affiliations

  1. Department of Medical Laboratory Science, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria

    Moses Agbomhere Hamed

  2. The Brainwill Laboratory, Osogbo, Osun State, Nigeria

    Moses Agbomhere Hamed, Folakemi Dorcas Fajuyitan, Omolade Christianah Adeyemo, Tomisin Folashade Odebunmi, Oluwapelumi Sarah Adeniyi & Isaac Ayomide Omole

  3. Reproductive Biology and Toxicology Research Laboratory, Oasis of Grace Hospital, Osogbo, Osun State, Nigeria

    Moses Agbomhere Hamed, Tunmise Maryanne Akhigbe, Isaac Ayomide Omole & Roland Eghoghosoa Akhigbe

  4. Department of Medical Microbiology and Parasitology, Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Nigeria

    Oyedele Oladipo Adegboyega & Olalekan Isreal Ojo

  5. Breeding and Genetics Unit, Department of Agronomy, Osun State University, Ejigbo Campus, Osun State, Nigeria

    Tunmise Maryanne Akhigbe

  6. Department of Microbiology, Faculty of Sciences, Federal University Oye-Ekiti, Oye-Ekiti, Ekiti State, Nigeria

    Folakemi Dorcas Fajuyitan & Tomisin Folashade Odebunmi

  7. Department of Microbiology, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, Osun State, Nigeria

    Omolade Christianah Adeyemo & Oluwapelumi Sarah Adeniyi

  8. Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria

    Isaac Ayomide Omole & Roland Eghoghosoa Akhigbe

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  1. Moses Agbomhere Hamed
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  2. Oyedele Oladipo Adegboyega
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  6. Omolade Christianah Adeyemo
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  10. Roland Eghoghosoa Akhigbe
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Correspondence to Roland Eghoghosoa Akhigbe.

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Hamed, M.A., Adegboyega, O.O., Ojo, O.I. et al. Glutamine-mediated Modulation of XO/uric acid/NF-kB Signaling Pathway Ameliorates Intestinal I/R-induced Bacterial Translocation and Cardiorenal Inflammatory Injury. Cell Biochem Biophys (2024). https://doi.org/10.1007/s12013-024-01252-6

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