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Protein Biomarkers in Serum of Patients with Schizophrenia

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Abstract

This study was devised to identify potential biomarkers of schizophrenia (SP) using proteomics techniques. We obtained 44 serum specimens from patients with SP, 26 specimens from patients with depression, and 40 specimens from healthy controls. Immobilized metal affinity capture protein chips (IMAC30) and surface-enhanced laser desorption-ionization time-of-flight mass spectrometry were used to isolate and obtain mass spectrometric data of differentially expressed serum proteins. The sequences of the peaks discrepant among the study groups were obtained using matrix-assisted laser desorption/ionization mass spectrometry and proteins identified using Mascot database. In the SP group, there were 91 protein peaks that were different from other study groups at the p value of <0.05 and 54 peaks different at the p value of <0.01. Two protein peaks at the mass-to-charge ratio of 1,207.41 and 1,466.78 were markedly different among the study groups, with the lowest expression in specimens from patients with SP. The amino acid sequences were, respectively, Glu-Gly-Asp-Phe-Leu-Ala-Glu-Gly-Gly-Gly-Val-Arg (EGDFLAEGGGVR) and Asp-Ser-Gly-Glu-Gly-Asp-Phe-Leu-Ala-Glu-Gly-Gly-Gly-Val-Arg (DSGEGDFLAEGGGVR). These proteins were identified as the N-terminal fragments of fibrinogen. In conclusion, these biomarker proteins may be useful for molecular diagnosis of SP.

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Abbreviations

SELDI-TOF–MS:

Surface-enhanced laser desorption/ionization, time-of-flight mass spectrometry

SPA:

Sinapinic acid

MALDI-TOF–MS:

Matrix assisted laser desorption/ionization, mass spectrometry

CV:

Coefficient of variance

m/z :

Mass-to-charge ratio

IMAC30:

Immobilized metal affinity surface

TFA:

Trifluoroacetic acid

EAM:

Energy absorption molecules

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Acknowledgments

The authors wish to thank the National High Technology Research and Development Program (Program 863, Grant Number 2006AA02090407) for providing funding for the study.

Conflict of interest

The authors declare no competing interests.

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    Authors and Affiliations

    1. Department of Laboratory Medicine, Affiliated Hospital of Luzhou Medical College, 25 Taiping Street, Luzhou, 646000, China

      Yin-huan Ding, Qiong-ying Hu, Wei Jiang & Kai-zheng Wang

    2. The People’s Hospital of Luzhou, 61 Zhongxiao Street, Luzhou, 646000, China

      Jian-hong Guo

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    1. Yin-huan Ding
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    3. Qiong-ying Hu
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    Correspondence to Kai-zheng Wang.

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    Yin-huan Ding and Jian-hong Guo contributed equally to this study and should be considered co-first authors.

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    Ding, Yh., Guo, Jh., Hu, Qy. et al. Protein Biomarkers in Serum of Patients with Schizophrenia. Cell Biochem Biophys 72, 799–805 (2015). https://doi.org/10.1007/s12013-015-0536-5

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