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Exploration on Mechanism of a New Type of Melatonin Receptor Agonist Neu-p11 in Hypoxia–Reoxygenation Injury of Myocardial Cells

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Abstract

To explore the mechanism of a new type of melatonin receptor agonist Neu-p11 in hypoxia–reoxygenation injury of myocardial cells. Hypoxia/reoxygenation (H/R) model of H9c2 myocardial cells was established, and the cells were divided into control group, H/R group, and Neu-p11 group. Apoptosis rates of myocardial cells in different groups, the contents of creatinine kinase (CK), lactic dehydrogenase (LDH), superoxide dismutase (SOD), and malondialdehyde (MDA) in cell culture media were compared. Myocardial cells in control group showed diverse shape, and the refractivity of cells were high and the pulse was strong with synchronous rhythm of 60–80/min; The refractivity of myocardial cells in H/R group decreased, the pseudopodium was thinner, and the rhythm was reduced to 30–40/min; The morphology and refractivity of myocardial cells in Neu-p11 group were significantly improved with rhythm of 50–60/min. The apoptosis rates in the control group, the H/R group, and the Neu-p11 group were 2.48, 39.66, and 17.94 %, respectively. Levels of CK, LDH, and MDA were significantly decreased in Neu-p11 compared with H/R group, yet, both of which were significantly higher than that in control group. The SOD level was significantly lower in H/R group compared to that in control group, and Neu-p11 group with no statistical difference between the Neu-p11 group and the control group. Neu-p11 has protective effects on hypoxia–reoxygenation injury of myocardial cells. It inhibits cell apoptosis and improves the morphology and rhythm of myocardial cells; It alleviates injury of cell membrane by reducing its permeability, which can stabilize myocardial cell membrane; It also alleviates lipid peroxidation and protects mitochondria from myocardial ischemia/reperfusion injury.

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Acknowledgments

This study was supported by the Research Foundation of Education Bureau of Zhejiang Province, China (Grant No. Y201328463).

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The authors have no conflicts of interest to declare.

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Authors and Affiliations

  1. Department of Cardiology, Zhejiang Provincial People’s Hospital, 158 Shangtang Road, Hangzhou, 310014, Zhejiang, China

    Jianwu Yu & Jing Wei

  2. Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou, 310058, Zhejiang, China

    Ling Ji & Xutao Hong

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  1. Jianwu Yu
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  2. Jing Wei
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Correspondence to Jianwu Yu.

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Yu, J., Wei, J., Ji, L. et al. Exploration on Mechanism of a New Type of Melatonin Receptor Agonist Neu-p11 in Hypoxia–Reoxygenation Injury of Myocardial Cells. Cell Biochem Biophys 70, 999–1003 (2014). https://doi.org/10.1007/s12013-014-0009-2

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