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Echocardiographic Evaluation of the Acute Cardiovascular Effects of an Endothelin-Like Peptide Extracted from the Venom of Atractaspis irregularis

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Abstract

Sarafotoxin-i3 from Atractaspis irregularis is a long sarafotoxin with an extended C terminus extension. Sarafotoxin-b from Atractaspis engaddensis is shorter by four amino acids. These peptides belong to the endothelin-like peptide family with a high sequence homology and similar three-dimensional structure. They act on endothelin receptors situated on the membrane of endothelial and smooth muscle cells. However, SRTX-i 3, despite a high toxicity, has a very low affinity for endothelin receptors compared to SRTX-b. The present work was carried out in order to compare the precise in vivo cardiovascular effect of SRTX-b and SRTX-i3. Male Wistar rats were anesthetized and mechanically ventilated. Doppler echocardiography was performed to measure left and right ventricular functions. The rats were divided into three groups that received intravenous injections of: saline, SRTX-b or SRTX-i3. All measurements were taken at baseline, at 1 min and at 6 min after injection. Both toxins impaired cardiac output. SRTX-b impaired left ventricular function, while SRTX-i3 increased airway pressures and led to acute right ventricular dilatation associated with a decreased tricuspid annulus peak systolic velocity. SRTX-b and SRTX-i3 appear to exert toxic effects via different mechanisms, SRTX-b impairs left ventricular function, while SRTX-i3 increases airway pressures and impairs right ventricular function.

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Abbreviations

SRTXs:

Sarafotoxins

SRTX-b:

Sarafotoxin-b extracted from the venom of Atractaspis engaddensis

SRTX-i3:

Sarafotoxin-i3 extracted from the venom of Atractaspis irregularis

ET-A:

Endothelin-receptor subtype A

ET-B:

Endothelin-receptor subtype B

LV:

Left ventricle

RV:

Right ventricle

LVFA:

Left ventricular fractional area

LVEDA:

Left ventricular end-diastolic area

RVEDA:

Right ventricular end-diastolic area

CO:

Cardiac output

SV:

Stroke volume

S′:

Tricuspid annulus peak systolic velocity

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Acknowledgments

We thank Prof. Michel Slama (Medical intensive care unit, CHU Amiens) for his helpful advices and Dr. Denis Servent (SIMOPRO-CEA-Saclay) for the participation of his research group.

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Authors and Affiliations

  1. Unité de réanimation chirurgicale, Pôle d’Anesthésie Réanimation et médecine d’urgence, CHU Amiens Nord, Place Victor Pauchet, 80054, Amiens cedex, France

    Yazine Mahjoub, Stéphanie Malaquin, Osama Abou Arab, Emmanuel Lorne, Alexis Salomon & Hervé Dupont

  2. Unité INSERM U1088, Amiens, France

    Yazine Mahjoub, Stéphanie Malaquin, Osama Abou Arab, Emmanuel Lorne, Alexis Salomon, Saïd Kamel & Hervé Dupont

  3. CEA, iMETI, Service d’Immuno Virologie (SIV), CEA Fontenay-aux-Roses, 92265, Fontenay-aux-Roses, France

    Frédéric Ducancel

  4. CEA, iBiTec-S, Service d’Ingénierie Moléculaire des Protéines (SIMOPRO), CEA Saclay, 91191, Gif sur Yvette, France

    Gilles Mourier

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  1. Yazine Mahjoub
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  2. Stéphanie Malaquin
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  9. Frédéric Ducancel
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Correspondence to Yazine Mahjoub.

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Mahjoub, Y., Malaquin, S., Abou Arab, O. et al. Echocardiographic Evaluation of the Acute Cardiovascular Effects of an Endothelin-Like Peptide Extracted from the Venom of Atractaspis irregularis . Cardiovasc Toxicol 17, 208–214 (2017). https://doi.org/10.1007/s12012-016-9376-9

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  • DOI: https://doi.org/10.1007/s12012-016-9376-9

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