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Selenium Counteracts Tight Junction Disruption and Attenuates the NF-κB-Mediated Inflammatory Response in Staphylococcus aureus-Infected Mouse Mammary Glands

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Abstract

Tight junctions (TJs) are the key determinant of barrier function in the mammary gland, with their disruption being associated with the pathogenesis and progression of mastitis, especially in the case of Staphylococcus aureus (S. aureus) infection. This study investigated whether selenium (Se) could attenuate S. aureus-induced mastitis by inhibiting inflammation and protecting mammary gland TJs in mice. The expression profiles of S. aureus-infected gland tissues derived from the gene expression omnibus dataset were analyzed. We found cytokine production, cell junctions, the nuclear transcription factor-κB (NF-κB) signalling pathway, and inflammatory responses associated with the differentially expressed genes, as revealed by Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment analyses. Se reduced the mRNA expression and production of inflammatory cytokines, including tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and decreased phosphorylation levels of the NF-κB complex. Moreover, Se alleviated structural damage and microvillus injury in mammary glands. Immunohistochemical staining revealed that Se increased the expression of Claudin-3; Western blot analysis revealed increased protein levels of Occludin and Tricellulin in the group supplemented with dietary Se. In summary, Se counteracted TJ disruption and attenuated NF-κB-mediated inflammatory responses in S. aureus-infected mouse mammary glands.

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The authors confirm that the data supporting the findings of this study are available within the article.

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Funding

This work was supported by the Hebei Province Graduate Innovation Funding Project (CXZZBS2023075), The Major Basic Program of Natural Science Foundation of Shandong Province, China (ZR2019ZD21), and the project of Shandong Province Higher Educational Outstanding Youth Innovation Team (2019KJF011).

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Authors and Affiliations

  1. College of Agriculture and Forestry Science, Linyi University, LinyiShandong Province, 276005, China

    Juan Wang, Shiyang XV & Chongliang Bi

  2. College of Animal Science and Technology, Hebei Agricultural University, BaodingHebei Province, 071001, China

    Junjun Liu

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Contributions

Chong-Liang Bi contributed to the overall study design and supervised all research. Junjun Liu carried out the experiments, analyzed the data, and was also responsible for the final editing of the manuscript. Juan Wang contributed partly to the writing and finally revising the manuscript and data analysis. Shiyang Xv drafted and revised the first version of the manuscript. All the authors reviewed and finally approved the manuscript.

Corresponding author

Correspondence to Chongliang Bi.

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The authors declare no competing interests.

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All the procedures of the study were performed under the approval of Laboratory Animals Research Center of Linyi Hebei province in P. R. China and the Ethics Committee of Linyi University and Hebei Agricultural University.

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The authors declare no competing interests.

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Junjun Liu and Juan Wang contributed equally to this work.

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Liu, J., Wang, J., XV, S. et al. Selenium Counteracts Tight Junction Disruption and Attenuates the NF-κB-Mediated Inflammatory Response in Staphylococcus aureus-Infected Mouse Mammary Glands. Biol Trace Elem Res (2024). https://doi.org/10.1007/s12011-024-04210-8

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