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Effectiveness of Selenium on Chondrocyte Glycoprotein Glycosylation Which Play Important Roles in the Pathogenesis of an Endemic Osteoarthritis, Kashin–Beck Disease

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Abstract

In this study, we aimed to explore the effectiveness of selenium on the chondrocyte glycoprotein glycosylation which plays important roles in the pathogenesis of Kashin–Beck disease (KBD). Cartilage samples were collected from KBD patients after total knee replacement surgery. Chondrocytes were cultured with sodium selenium. The group of chondrocytes which were cultured without adding sodium selenium was considered as control group. Lectin microarray was used to screen the differences in lectin levels between KBD and KBD with selenium groups. Stronger signals for Bandeiraea simplicifolia (BS-I), Hippeastrum hybrid lectin (HHL), Pisum sativum agglutinin (PSA), Psophocarpus tetragonolobus lectin I (PTL-I), Psophocarpus tetragonolobus lectin II (PTL-II), Sophora japonica agglutinin (SJA), Lotus tetragonolobus lectin (LTL), and Triticum vulgaris (WGA) were observed in the KBD group. Meanwhile, Aleuria aurantia lectin (AAL), Lens culinaris agglutinin (LCA), Lycopersicon esculentum (tomato) lectin (LEL), Peanut agglutinin (PNA), and Sambucus nigra lectin (SNA) signals were lower in the KBD group. Selenium may have the function of influence the expression levels of carbohydrate chains Galα1,3-Gal, high mannose, and GlcNAc.

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Data Availability

The data of this study will be made available on reasonable request.

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Funding

This study was supported by Shaanxi Natural Science Basic Research Projects (2021JM-037 and 2019JM-486), the National Natural Science Foundation of China key international cooperation project (81620108026).

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Author notes

  1. Sen Wang and Lingling Geng contributed equally to this work.

Authors and Affiliations

  1. School of Public Health, Health Science Center, Xi’an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission, Xi’an, Shaanxi, China

    Sen Wang, Peilin Meng, Linlin Yuan & Xiong Guo

  2. Department of Rheumatology and Immunology, Xi’an Children’s Hospital, Xi’an, Shaanxi Province, China

    Lingling Geng

  3. Xi’an Honghui Hospital, Health Science Center, Xi’an Jiaotong University, Xi’an, Shaanxi, China

    Guanghui Zhao

Authors
  1. Sen Wang
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  2. Lingling Geng
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  3. Guanghui Zhao
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  4. Peilin Meng
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  5. Linlin Yuan
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  6. Xiong Guo
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Contributions

Sen Wang did the experiments and wrote the manuscript; Lingling Geng did the experiments and revised the manuscript; Guanghui Zhao analyzed the experiment data; Peiling Meng and Linlin Yuan collected the cartilage. Xiong Guo designed the project.

Corresponding author

Correspondence to Xiong Guo.

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Ethical Approval

Informed consent was obtained from each subject involved in the investigation. This research was approved by the Human Ethics Committee of Xi’an Jiaotong University, Xi’an, Shaanxi, China, and conducted in accordance with the 1964 Helsinki Declaration.

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The authors declare no competing interests.

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Wang, S., Geng, L., Zhao, G. et al. Effectiveness of Selenium on Chondrocyte Glycoprotein Glycosylation Which Play Important Roles in the Pathogenesis of an Endemic Osteoarthritis, Kashin–Beck Disease. Biol Trace Elem Res 200, 1531–1537 (2022). https://doi.org/10.1007/s12011-021-02778-z

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