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The Chronic Use of Magnesium Decreases VEGF Levels in the Uterine Tissue in Rats

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Abstract

Vascular endothelial growth factor (VEGF) is the most important regulator of angiogenesis which serves to provide sufficient blood supply, and can trigger both physiological and pathological angiogenesis. Recent studies have shown that VEGF increases in gynecological diseases (such as endometriosis, ovarian, and endometrial cancers) and is a prognostic factor in these pathologies. Therefore, VEGF should be maintained at appropriate levels. Magnesium is used in many gynecological practices (such as eclampsia, preeclampsia, dysmenorrhea, and climacteric symptoms) and the mechanisms of action are still under investigation. Redox status, which can be regulated by magnesium, was shown to affect VEGF expression. The aim of this study was to evaluate the effects of chronic magnesium use on VEGF and oxidative status in the uterus. Magnesium sulfate was administered to rats at doses of 30 mg/kg (intramuscular) for 2 weeks. VEGF, Superoxide dismutase (SOD), Glutathione peroxidase (GPx), and Malondialdehyde (MDA) levels were measured using ELISA; vascular and cellular alterations were determined by histology in the uterine tissue at the metoestrus phase. In the uterine tissue of Mg-treated subjects, magnesium levels increased while VEGF, SOD, GPx, and MDA levels decreased without histological changes. There was a negative correlation between uterine tissue magnesium levels and VEGF, SOD, GPx, and MDA levels. Consequently, the results of this study demonstrated that regular magnesium use decreased VEGF levels in uterus. Decreased VEGF levels were associated with decreased uterine oxidative stress. Chronic magnesium usage may protect the uterine tissue from certain diseases in which angiogenesis is critical.

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Authors and Affiliations

  1. Department of Physiology, School of Medicine, Dokuz Eylul University, Balçova, Izmir, Turkey

    Ferda Hoşgörler, Başar Koç, Sevim Kandis, Güven Güvendi, Rabia Ilgin & Nazan Uysal

  2. College of Vocational School of Health Services, School of Medicine, Dokuz Eylul University, Izmir, Turkey

    Servet Kızıldağ & Mehmet Ateş

  3. Department of Pathology, Bozyaka Training and Research Hospital, Izmir, Turkey

    Asuman Argon

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  1. Ferda Hoşgörler
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  2. Servet Kızıldağ
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Correspondence to Nazan Uysal.

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The experiments were carried out according to the guiding principles in the use of experimental animals and approved by the Animal Care and Use Committee of the Dokuz Eylül University, School of Medicine.

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Hoşgörler, F., Kızıldağ, S., Ateş, M. et al. The Chronic Use of Magnesium Decreases VEGF Levels in the Uterine Tissue in Rats. Biol Trace Elem Res 196, 545–551 (2020). https://doi.org/10.1007/s12011-019-01944-8

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