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The Protective Role of Selenium in AFB1-Induced Tissue Damage and Cell Cycle Arrest in Chicken’s Bursa of Fabricius

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Abstract

Aflatoxin B1 (AFB1) is a naturally occurring secondary metabolites of Aspergillus flavus and Aspergillus parasiticus, and is the most toxic form of aflatoxins. Selenium (Se) with antioxidant and detoxification functions is one of the essential trace elements for human beings and animals. This study aims to evaluate the protective effects of Se on AFB1-induced tissue damage and cell cycle arrest in bursa of Fabricius (BF) of chickens. The results showed that a dietary supplement of 0.4 mg·kg−1 Se alleviated the histological lesions induced by AFB1, as demonstrated by decreasing vacuoles and nuclear debris, and relieving oxidative stress. Furthermore, flow cytometry studies showed that a Se supplement protected AFB1-induced G2M phase arrest at 7 days and G0G1 phase arrest at 14 and 21 days. Moreover, the mRNA expression results of ATM, Chk2, p53, p21, cdc25, PCNA, cyclin D1, cyclin E1, cyclin B3, CDK6, CDK2, and cdc2 indicated that Se supplement could restore these parameters to be close to those in the control group. It is concluded that a dietary supplement of 0.4 mg kg−1 Se could diminish AFB1-induced immune toxicity in chicken’s BF by alleviating oxidative damage and cell cycle arrest through an ATM-Chk2-cdc25 route and the ATM-Chk2-p21 pathway.

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Funding

This work was funded by the program for the Changjiang Scholars and University Innovative Research Team (IRT 0848) and the Science and Technology Department of Sichuan Province (2012FZ0066 and 2013FZ0072) as well as the Huimin Project of Chengdu Science and Technology (2016-HM01-00337-SF).

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  1. Ping Hu, Zhicai Zuo and Fengyuan Wang contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, People’s Republic of China

    Ping Hu, Zhicai Zuo, Fengyuan Wang, Ke Guan, Hang Li, Jing Fang & Hengmin Cui

  2. College of Veterinary Medicine Chengdu, Sichuan Agricultural University, Chengdu, 611130, Sichuan, People’s Republic of China

    Ke Guan, Hang Li, Jing Fang, Hengmin Cui, Gang Su & Ping Ouyang

  3. College of Life Sciences, China West Normal University, Nanchong, 637002, Sichuan, People’s Republic of China

    Xi Peng

  4. Life Science Department, Sichuan Agricultural University, Ya’an, 625014, Sichuan, People’s Republic of China

    Yi Zhou

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  1. Ping Hu
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Correspondence to Xi Peng or Jing Fang.

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All procedures performed in studies involving animals were in accordance with the international guidelines and the laws and ethical standards of the Sichuan Agricultural University Animal Care and Use Committee (Approval No. 2012-024) where the studies were conducted.

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Hu, P., Zuo, Z., Wang, F. et al. The Protective Role of Selenium in AFB1-Induced Tissue Damage and Cell Cycle Arrest in Chicken’s Bursa of Fabricius. Biol Trace Elem Res 185, 486–496 (2018). https://doi.org/10.1007/s12011-018-1273-6

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