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Improved Sp1 and Betaine Homocysteine-S-Methyltransferase Expression and Homocysteine Clearance Are Involved in the Effects of Zinc on Oxidative Stress in High-Fat-Diet-Pretreated Mice

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Abstract

Zinc plays a role in alleviating oxidative stress. However, the related mechanisms remain to be further elucidated. The present study was conducted to investigate whether the recovery of oxidative stress in high-fat-diet (HFD)-pretreated mice was affected by zinc. Male mice received either an HFD or a low-fat-diet (LFD) for 8 weeks. Then, the mice fed with HFD and LFD were both assigned to either a control diet (30 mg zinc, ZD) or a no-added zinc diet (NZD) for an additional 4 weeks. The results showed that after feeding with NZD for 4 weeks, the HFD-pretreated mice had the highest plasma glucose and insulin concentrations, while had the lowest CuZn-SOD and glutathione concentrations. Moreover, after feeding with NZD for 4 weeks, the HFD-pretreated mice had the highest hepatic ROS and homocysteine concentrations, while had the lowest glutathione and methionine concentrations. Furthermore, the HFD-pretreated mice fed with NZD for 4 weeks had the lowest gene and protein expression of betaine homocysteine-S-methyltransferase (BHMT), cystathionine β-synthase, and Sp1. The results suggested that zinc was critical for oxidative stress alleviation and homocysteine clearance in HFD-pretreated mice. It was further elucidated that improved Sp1 and BHMT expression are involved in the effects of zinc on oxidative stress.

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Funding

This work is supported by grants from the National Basic Research Program of China (No. 2013CB127301) and National Key Research and Development Program of China (No. 2016YFD0501201; 2016YFD0500504).

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Correspondence to Xihong Zhou or Qinghua He.

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The study was conducted according to the principles of the animal welfare committee of the Institute of Subtropical Agriculture, Chinese Academy of Sciences and was approved by the animal welfare committee of the Institute of Subtropical Agriculture, Chinese Academy of Sciences.

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The authors declare that they have no conflict of interest.

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Informed consent was obtained from all individual participants included in the study.

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Wu, L., Zhou, X., Li, T. et al. Improved Sp1 and Betaine Homocysteine-S-Methyltransferase Expression and Homocysteine Clearance Are Involved in the Effects of Zinc on Oxidative Stress in High-Fat-Diet-Pretreated Mice. Biol Trace Elem Res 184, 436–441 (2018). https://doi.org/10.1007/s12011-017-1214-9

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  • DOI: https://doi.org/10.1007/s12011-017-1214-9

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