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The Effect of Chronic Fluorosis on Calcium Ions and CaMKIIα, and c-fos Expression in the Rat Hippocampus

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Abstract

This study investigated neurotoxicity of chronic fluorosis in the rat hippocampus. Newly weaning, male, Sprague-Dawley (SD) rats were administered 15, 30, and 60 mg/L sodium fluoride (NaF) solution (fluorine ion concentration 8.25, 16.50, and 33.00 mg/L, respectively), and tap water, for 18 months. The neurotoxicological mechanism was examined with a focus on intracellular calcium overload. Results showed that as the fluoride concentration increased, calcium ion concentration [Ca2+], the expression of calcium/calmodulin-dependent protein kinase II α (CaMKIIα), and the expression of catus proto-oncogene protein c-fos (c-fos) all tend to increase. Compared to the control group, Ca2+, CaMKIIα, and c-fos significantly increased (P < 0.05) in the moderate-fluoride and the high-fluoride groups. These results indicate that Ca2+/CaMKIIα/c-fos channel signal may be the molecular mechanism of central nervous system damage caused by chronic fluoride intoxication. Moreover, elevated Ca2+ concentration in the hippocampus may be the initiating factor of neuronal apoptosis induced by fluoride.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (grant number, 815773101).

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Authors and Affiliations

  1. College of Environmental Science and Engineering, Qingdao University, Qingdao, Shandong, China

    Yao Teng & Juan Feng

  2. College of Chemistry and Life Science, Qingdao Technical College, Qiantangjiang Road, Qingdao, Shandong, 266555, China

    Jing Zhang

  3. Laboratory of Pollution Ecology, Xingzhi College, Zhejiang Normal University, Yingbin Avenue, Jinhua, Zhejiang, 321004, China

    Zigui Zhang

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  1. Yao Teng
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Correspondence to Jing Zhang or Zigui Zhang.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Informed consent was obtained from all individual participants included in the study.

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Teng, Y., Zhang, J., Zhang, Z. et al. The Effect of Chronic Fluorosis on Calcium Ions and CaMKIIα, and c-fos Expression in the Rat Hippocampus. Biol Trace Elem Res 182, 295–302 (2018). https://doi.org/10.1007/s12011-017-1098-8

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