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Apocynin Alleviates Renal Ischemia/Reperfusion Injury Through Regulating the Level of Zinc and Metallothionen

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Abstract

The purpose of this research was to evaluate the protective effects of apocynin on renal ischemia/reperfusion (I/R) injury (RI/RI) in rats. Rats preconditioned with apocynin were subjected to renal I/R. Zinc levels in serum and renal tissues, blood urea nitrogen (BUN), and serum creatinine (Scr) were detected. We further measured the activity of superoxide dismutase (SOD); the content of malondialdehyde (MDA), IL-4, IL-6, IL-10, and TNF-α; and the expression of metallothionein (MT) in the renal tissues. Results indicated that the levels of MDA, IL-4, IL-6, IL-10, TNF-α, and MT in the kidney tissue and serum BUN and Scr levels in RI/RI group were significantly higher than those in sham-operated group, while the levels of serum Zn and kidney Zn and SOD were reduced in RI/RI group. Apocynin treatment further decreased the levels of MDA, IL-6, TNF-α, and serum BUN and Scr, whereas it significantly increased the levels of Zn, SOD, IL-4, IL-10, and MT in the kidney tissue and serum Zn. These findings suggest that apocynin might play a protective role against RI/RI in rats through regulating zinc level and MT expression involving in oxidative stress.

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Acknowledgements

The authors appreciate the support from the financial support from the Science and Technology Planning Project of Zhejiang Province (2015C37130), the Zhejiang Provincial Natural Science Foundation (LQ14H230001), and the Science and Technology Planning Project of Jiaxing (2012AY1072-3).

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Authors and Affiliations

  1. Department of Pathology and Nephrology, Jiaxing Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, 314001, Jiaxing, Zhejiang Province, People’s Republic of China

    Bo Hu, Yuhong Wu, Jie Liu, Xiaohua Shen & Ruilin Shen

  2. Department of Pathology, Provincial Key Discipline of Pharmacology, Jiaxing University Medical College, 314001, Jiaxing, Zhejiang Province, People’s Republic of China

    Fei Tong & Guangtao Xu

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  1. Bo Hu
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  2. Yuhong Wu
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Correspondence to Guangtao Xu.

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Bo Hu and Yuhong Wu contributed equally to this work.

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Hu, B., Wu, Y., Tong, F. et al. Apocynin Alleviates Renal Ischemia/Reperfusion Injury Through Regulating the Level of Zinc and Metallothionen. Biol Trace Elem Res 178, 71–78 (2017). https://doi.org/10.1007/s12011-016-0904-z

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