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The Synergistic Effect of Serine with Selenocompounds on the Expression of SelP and GPx in HepG2 Cells

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Abstract

We explored the synergistic effect of serine combined with several selenocompounds or used alone on the expression of selenoprotein P (SelP) and glutathione peroxidase (GPx) in this study. We first compared the SelP and GPx expression difference between HepG2 and Hela cells treated with serine and finally chose HepG2 as experimental cell. In the serine-used-alone experiment, three kinds of selenium nutritional models (low-, adequate-, and high-selenium) were established and serine was 10 times gradient diluted (0.01 to 100 μmol/L). In the combined experiment, the selenocompound doses were set as 0.01, 0.1, and 1 μmol Se/L and serine was set according to its molar ratio with the selenocompounds. We found that SelP and GPx concentrations in the low-, adequate-, and high-selenium models increased following with serine dose. When the concentration of sodium selenite and SeMet was 1 μmol Se/L while MeSeCys was 0.1 and 1 μmol Se/L, SelP concentrations for serine combined with selenocompounds groups were significantly higher than that of selenocompounds used alone. When the concentration of sodium selenite was 0.1 μmol Se/L, SeMet was 0.1 and 1 μmol Se/L while MeSeCys was 0.01 and 1 μmol Se/L, GPx concentrations for serine combined with selenocompounds groups were significantly higher than that of selenocompounds used alone. Our preliminary result indicated the beneficial effect of serine on the expression of SelP and GPx, which suggested that it might be a candidate for combined selenium supplement.

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Acknowledgments

All authors read and approved the final manuscript. This study was financially supported by the National Natural Science Foundation of China under the Grant No. 81372989. Q. W. specially thanks Prof. Y. Hu at the Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Chinese Academy of Sciences, for helpful discussions. She also acknowledges hospitality of the staff there during her recent visit to the laboratory.

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  1. National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, 100050, China

    Qin Wang, Li-cui Sun, Yi-qun Liu, Jia-xi Lu, Feng Han & Zhen-wu Huang

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  1. Qin Wang
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Correspondence to Zhen-wu Huang.

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Wang, Q., Sun, Lc., Liu, Yq. et al. The Synergistic Effect of Serine with Selenocompounds on the Expression of SelP and GPx in HepG2 Cells. Biol Trace Elem Res 173, 291–296 (2016). https://doi.org/10.1007/s12011-016-0665-8

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